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1. (WO2019063849) PURIFICATION OF A POLYPEPTIDE OF INTEREST
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Pub. No.: WO/2019/063849 International Application No.: PCT/EP2018/085532
Publication Date: 04.04.2019 International Filing Date: 18.12.2018
IPC:
C07K 1/22 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
16
by chromatography
22
Affinity chromatography or related techniques based upon selective absorption processes
Applicants:
DSM IP ASSETS B.V. [NL/NL]; Het Overloon 1 6411 TE HEERLEN, NL
Inventors:
FERREIRA DE PASSOS CORREIA, Guilherme, Nuno; NL
DAMVELD, Robbertus, Antonius; NL
PEREIRA GALO NEVES, Catarina; NL
Agent:
DSM INTELLECTUAL PROPERTY; P.O. Box 4 6100 AA ECHT, NL
Priority Data:
17209005.220.12.2017EP
Title (EN) PURIFICATION OF A POLYPEPTIDE OF INTEREST
(FR) PURIFICATION D'UN POLYPEPTIDE D'INTÉRÊT
Abstract:
(EN) The present invention relates to the field of microbiology. More specifically, the present invention relates to a method for purification of an enzyme in a composition comprising the enzyme and further comprising a nucleic acid, said method comprises (i) incubating the composition with an anion exchange agent under conditions where the enzyme does not bind to the anion exchange agent while the nucleic acid does bind to the anion exchange agent, and (ii) separating the composition comprising the enzyme from anion exchange agent comprising the nucleic acid to yield a purified composition comprising the enzyme.
(FR) La présente invention concerne le domaine de la microbiologie. Plus spécifiquement, la présente invention concerne un procédé de purification d'une enzyme dans une composition comprenant l'enzyme et comprenant en outre un acide nucléique, ledit procédé consistant à (i) incuber la composition avec un agent d'échange d'anions dans des conditions dans lesquelles l'enzyme ne se lie pas à l'agent d'échange d'anions tandis que l'acide nucléique se lie à l'agent d'échange d'anions, et (ii) séparer la composition comprenant l'enzyme d'un agent d'échange d'anions comprenant l'acide nucléique pour produire une composition purifiée comprenant l'enzyme.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)