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1. (WO2019063839) CMV RESISTANCE ALLELE
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Pub. No.: WO/2019/063839 International Application No.: PCT/EP2018/076640
Publication Date: 04.04.2019 International Filing Date: 01.10.2018
IPC:
C07K 14/415 (2006.01) ,A01H 1/04 (2006.01) ,A01H 5/12 (2018.01) ,A01H 6/02 (2018.01) ,C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415
from plants
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1
Processes for modifying genotypes
04
Processes of selection
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
12
Leaves
[IPC code unknown for A01H 6/02]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. [NL/NL]; Burgemeester Crezeelaan 40 2678 KX De Lier, NL
Inventors:
KOCK, Vincent Laurens Adrianus; NL
FEITSMA, Johannes Geert Jan; NL
FRIJTERS, Raoul Jacobus Johannes Maria; NL
Agent:
VAN SOMEREN, Petronella Francisca Hendrika Maria; NL
Priority Data:
15/720,75729.09.2017US
PCT/EP2017/07486229.09.2017EP
Title (EN) CMV RESISTANCE ALLELE
(FR) ALLÈLE DE RÉSISTANCE AU CMV
Abstract:
(EN) The present invention relates to an allele designated alpha-CMV which confers resistance to CMV when present in a spinach plant, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif "MAEIGYSVC" at its N-terminus; and b) the motif "KWMCLR"; and c) an LRR domain that has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele has a genomic nucleotide sequence which in order of increased preference has at least 80%, 81%, 82%, 83%, 84%,85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:1. The invention further relates to plants comprising the allele and to various methods involving the allele.
(FR) La présente invention concerne un allèle désigné alpha-CMV qui confère une résistance au CMV une fois présent dans une plante d'épinard, la protéine codée par ledit allèle étant une protéine CC-NBS-LRR qui comprend dans sa séquence d'acides aminés : a) le motif " MAEIGYSVC " à son extrémité N-terminale; et b) le motif " KWMCLR "; et c) un domaine LRR ayant dans l'ordre de préférence croissant au moins 95 %, 96 %, 97 %, 98 %, 99 %, 100 % de similarité de séquence avec SEQ ID No : 10. L'allèle a une séquence nucléotidique génomique qui, dans l'ordre de préférence croissant, présente au moins 80 %, 81 %, 82 %, 83 %, 84 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 %, 99 %, 100 % de similarité de séquence avec SEQ ID No : 1. L'invention concerne en outre des plantes comprenant l'allèle et divers procédés impliquant l'allèle.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)