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1. (WO2019063757) PROCESS FOR THE PURIFICATION OF A NEUTRAL HUMAN MILK OLIGOSACCHARIDE (HMO) FROM MICROBIAL FERMENTATION
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Pub. No.: WO/2019/063757 International Application No.: PCT/EP2018/076392
Publication Date: 04.04.2019 International Filing Date: 28.09.2018
IPC:
C07H 1/08 (2006.01) ,C07H 3/06 (2006.01) ,C12P 19/14 (2006.01) ,A23L 29/30 (2016.01) ,A23L 33/00 (2016.01) ,A23L 33/21 (2016.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1
Processes for the preparation of sugar derivatives
06
Separation; Purification
08
from natural products
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3
Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06
Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
14
produced by the action of a carbohydrase, e.g. by alpha-amylase
[IPC code unknown for A23L 29/30][IPC code unknown for A23L 33][IPC code unknown for A23L 33/21]
Applicants:
FRIESLANDCAMPINA NEDERLAND B.V. [NL/NL]; Stationsplein 4 3818 LE Amersfoort, NL
Inventors:
DIJKSTRA, Zwaantje Johanna; NL
JEURISSEN, Franciscus Johannes Hubertus; NL
VAN GENUCHTEN, Martina Henrica Maria; NL
HUISMAN, Jacob; NL
Agent:
FRIESLANDCAMPINA NEDERLAND B.V.; Bronland 20 P.O. Box 238 6700 AE Wageningen, NL
Priority Data:
17194188.329.09.2017EP
Title (EN) PROCESS FOR THE PURIFICATION OF A NEUTRAL HUMAN MILK OLIGOSACCHARIDE (HMO) FROM MICROBIAL FERMENTATION
(FR) PROCÉDÉ DE PURIFICATION D'UN OLIGOSACCHARIDE NEUTRE DE LAIT HUMAIN (HMO) À PARTIR DE FERMENTATION MICROBIENNE
Abstract:
(EN) The invention relates to a process for the purification of a neutral human milk oligosaccharide (HMO) from a fermentation broth, the process comprising the steps of: (i) separating biomass from the fermentation broth to provide a crude solution; (ii) treating the crude solution with: (a) a cation- exchange material; (b) an anion-exchange material; and (c) a cation-exchange adsorbent resin; thereby obtaining a purified solution containing the neutral human milk oligosaccharide. Further, the invention relates to a process for fermentatively producing HMO in a fermentation broth and purifying the HMO from the broth.
(FR) L'invention concerne un procédé de purification d'un oligosaccharide neutre de lait humain (HMO) à partir d'un bouillon de fermentation, le procédé comprenant les étapes consistant à : (i) séparer la biomasse du bouillon de fermentation pour obtenir une solution brute ; (ii) traiter la solution brute avec : (a) un matériau échangeur de cations ; (b) un matériau échangeur d'anions ; et (c) une résine adsorbante échangeuse de cations ; ce qui permet d'obtenir une solution purifiée contenant l'oligosaccharide neutre de lait humain. L'invention concerne en outre, un procédé de production de HMO par fermentation dans un bouillon de fermentation, et de purification du HMO à partir du bouillon.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)