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1. (WO2019063507) IMPROVED PROCESS FOR ETHANOL PRODUCTION
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/063507 International Application No.: PCT/EP2018/075866
Publication Date: 04.04.2019 International Filing Date: 25.09.2018
IPC:
C12P 7/06 (2006.01) ,C12N 9/12 (2006.01) ,C12N 9/02 (2006.01) ,C12N 9/10 (2006.01) ,C12N 9/04 (2006.01)
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
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Ethanol, i.e. non-beverage
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
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Transferases (2.)
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transferring phosphorus containing groups, e.g. kinases (2.7)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
Applicants:
DSM IP ASSETS B.V. [NL/NL]; Het Overloon 1 6411 TE HEERLEN, NL
Inventors:
DE WAAL, Paulus Petrus; NL
VUGT-VAN LUTZ, Ingrid Maria; NL
SCHMITZ, Jozef Petrus Johannes; NL
DE BRUIJN, Hans Marinus Charles Johannes; NL
Agent:
DSM INTELLECTUAL PROPERTY; P.O. Box 4 6100 AA ECHT, NL
Priority Data:
17193039.926.09.2017EP
Title (EN) IMPROVED PROCESS FOR ETHANOL PRODUCTION
(FR) PROCÉDÉ AMÉLIORÉ POUR LA PRODUCTION D'ÉTHANOL
Abstract:
(EN) The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less. A recombinant yeast having the genes as described above is particularly sensitive towards acetic acid, and the ethanol yield rapidly decreases when the composition contains more than 10 mM undissociated acetic acid.
(FR) L'invention concerne un procédé de production d'éthanol à partir d'une composition comprenant au moins du glucose comprenant la fermentation de ladite composition en présence d'une levure recombinante ; et la récupération de l'éthanol, ladite levure comprenant un ou plusieurs gènes codant pour une enzyme ayant une activité glycérol déshydrogénase, un ou plusieurs gènes codant pour une enzyme ayant une activité dihydroxyacétone kinase (EC 2.7.1.28 et/ou EC 2.7.1.29) ; un ou plusieurs gènes codant pour une enzyme dans une voie de production d'acétyl-CoA et un ou plusieurs gènes codant pour une enzyme ayant au moins une activité acétaldéhyde déshydrogénase dépendante de NAD + (EC 1.2.1.10 ou EC 1.1.1.2), et éventuellement un ou plusieurs gènes codant pour un transporteur de glycérol, la composition comprenant une quantité d'acide acétique non dissocié de 10 mM ou moins. Une levure recombinante ayant les gènes tels que décrits ci-dessus est particulièrement sensible à l'acide acétique, et le rendement en éthanol diminue rapidement lorsque la composition contient plus de 10 mM d'acide acétique non dissocié.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)