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1. (WO2019062895) USE OF MAIZE GENE ZMABCG20 IN REGULATING CROP MALE FERTILITY AND DNA MOLECULAR MARKERS ASSOCIATED WITH MAIZE MALE FERTILITY AND USE THEREOF
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Pub. No.: WO/2019/062895 International Application No.: PCT/CN2018/108583
Publication Date: 04.04.2019 International Filing Date: 29.09.2018
IPC:
C12N 15/82 (2006.01) ,C12N 15/29 (2006.01) ,C07K 14/415 (2006.01) ,A01H 5/00 (2018.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
29
Genes encoding plant proteins, e.g. thaumatin
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415
from plants
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
海南波莲水稻基因科技有限公司 HAINAN BOLIAN RICE GENE TECHNOLOGY CO., LTD. [CN/CN]; 中国海南省海口市 紫荆路2-1号紫荆信息公寓26A 26A Zijing Xinxi Mansion, No. 2-1 Zijing Road Haikou, Hainan 570125, CN
Inventors:
黄培劲 HUANG, Peijin; CN
李新鹏 LI, Xinpeng; CN
李京琳 LI, Jinglin; CN
陈磊 CHEN, Lei; CN
李燕群 LI, Yanqun; CN
陈江淑 CHEN, Jiangshu; CN
陈思兰 CHEN, Silan; CN
安保光 AN, Baoguang; CN
龙湍 LONG, Tuan; CN
吴永忠 WU, Yongzhong; CN
Agent:
北京路浩知识产权代理有限公司 CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED; 中国北京市 海淀区丹棱街3号中国电子大厦B座18层 18th Floor Tower B, CEC Plaza, No. 3 Dan Ling Street, Haidian District Beijing 100080, CN
Priority Data:
201710919712.730.09.2017CN
201710919714.630.09.2017CN
Title (EN) USE OF MAIZE GENE ZMABCG20 IN REGULATING CROP MALE FERTILITY AND DNA MOLECULAR MARKERS ASSOCIATED WITH MAIZE MALE FERTILITY AND USE THEREOF
(FR) UTILISATION DU GÈNE ZMABCG20 DU MAÏS DANS LA RÉGULATION DE LA FERTILITÉ MÂLE DE CULTURE ET MARQUEURS MOLÉCULAIRES D'ADN ASSOCIÉS À LA FERTILITÉ MÂLE DU MAÏS ET UTILISATION CORRESPONDANTE
(ZH) 玉米基因ZmABCG20在调控作物雄性育性中的应用以及与玉米雄性生育力相关的DNA分子标记及其应用
Abstract:
(EN) Provided is a use of maize gene ZmABCG20 in regulating male fertility of crops. The genomic DNA sequence of ZmABCG20 in maize variety B73 is shown by SEQ ID NO: 1, and the encoded protein sequence is shown in SEQ ID NO: 3. Also provided is a mutant zmabcg20-1 of the gene ZmABCG20 and use thereof, and the mutated gene sequence is shown in SEQ ID NO: 7. Also provided is a molecular marker identification method of the mutated gene. Also provided is a DNA molecular marker associated with male fertility in maize, the DNA molecule marker being located at bases 326-329 after the start codon of the maize gene ZmABCG20 nucleic acid sequence, and the sequence is TGCA, and the 4 base deleted maize line exhibits recessive male sterility. The DNA molecular marker of the present invention can be used to identify or breed male sterile maize germplasm resources and the like.
(FR) L'invention concerne l'utilisation du gène ZmABCG20 du maïs dans la régulation de la fertilité mâle de cultures. La séquence d'ADN génomique de ZmABCG20 dans la variété de maïs B73 est représentée par la séquence SEQ ID NO:1 et la séquence de la protéine codée est représentée dans la séquence SEQ ID NO:3. L'invention concerne également un mutant zmabcg20-1 du gène ZmABCG20 et une utilisation correspondante et la séquence génique mutée est représentée dans la séquence SEQ ID NO:7. L'invention concerne un procédé d'identification de marqueurs moléculaires du gène muté. L'invention concerne également un marqueur moléculaire d'ADN associé à la fertilité mâle dans le maïs, le marqueur moléculaire d'ADN étant situé au niveau des bases 326-329 en aval du codon de départ de la séquence d'acide nucléique du gène de maïs ZmABCG20 et la séquence est TGCA et la lignée de maïs à 4 bases supprimées présente une stérilité mâle récessive. Le marqueur moléculaire d'ADN de la présente invention peut être utilisé pour identifier ou reproduire des ressources de matériel génétique de maïs stérile mâle et analogues.
(ZH) 玉米基因ZmABCG20在调控作物雄性育性中的应用,玉米品种B73中ZmABCG20的基因组DNA序列如SEQ ID NO:1所示,所编码的蛋白序列如SEQ ID NO:3所示。基因ZmABCG20的突变体zmabcg20-1及其应用,突变基因序列如SEQ ID NO:7所示;还提供了该突变基因的分子标记鉴定方法。与玉米雄性生育力相关的DNA分子标记,所述DNA分子标记位于玉米基因ZmABCG20核酸序列起始密码子后第326-329位碱基,序列为TGCA,该4个碱基缺失的玉米品系表现出隐性雄性核不育性状。本发明的DNA分子标记可用于鉴定或选育雄性不育玉米种质资源等。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)