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1. (WO2019062887) METHOD FOR GENERATING CIRCULAR NUCLEIC ACID MOLECULES IN VITRO
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/062887 International Application No.: PCT/CN2018/108557
Publication Date: 04.04.2019 International Filing Date: 29.09.2018
IPC:
C12N 15/85 (2006.01) ,C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
中国科学院动物研究所 INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES [CN/CN]; 中国北京市 朝阳区北辰西路1号院5号 No.5, 1 Beichen West Road, Chaoyang District Beijing 100101, CN
Inventors:
王皓毅 WANG, Haoyi; CN
程晨 CHENG, Chen; CN
唐娜 TANG, Na; CN
李佳鑫 LI, Jiaxin; CN
操时伟 CAO, Shiwei; CN
Agent:
北京永新同创知识产权代理有限公司 NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD.; 中国北京市 东城区北三环东路36号北京环球贸易中心C座10层 10th Floor Tower C, Beijing Global Trade Center, 36 North Third Ring Road East, Dongcheng District Beijing 100013, CN
Priority Data:
201710917265.130.09.2017CN
Title (EN) METHOD FOR GENERATING CIRCULAR NUCLEIC ACID MOLECULES IN VITRO
(FR) PROCÉDÉ DE GÉNÉRATION DE MOLÉCULES D'ACIDE NUCLÉIQUE CIRCULAIRE IN VITRO
(ZH) 一种体外产生环状核酸分子的方法
Abstract:
(EN) Provided is a method for generating circular nucleic acid molecules in vitro. Specifically, provided is an in-vitro method for efficiently generating minicircle DNA without depending on bacteria. The present invention also relates to minicircle DNA generated by using the method, and uses thereof.
(FR) L'invention concerne un procédé de génération de molécules d'acide nucléique circulaire in vitro. De façon spécifique, l'invention concerne un procédé in vitro pour générer efficacement un ADN mini-circulaire sans dépendre de bactéries. La présente invention concerne également de l'ADN semi-circulaire généré par le procédé et ses utilisations.
(ZH) 提供一种体外产生环状核酸分子的方法。具体而言,提供一种不依赖于细菌而高效产生minicircle DNA的体外方法,还涉及通过该方法产生的minicircle DNA及其用途。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)