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1. (WO2019062709) RECOMBINANT BOVINE SERUM ALBUMIN MATURE PEPTIDE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
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Pub. No.: WO/2019/062709 International Application No.: PCT/CN2018/107336
Publication Date: 04.04.2019 International Filing Date: 25.09.2018
IPC:
C07K 14/765 (2006.01) ,C12N 15/12 (2006.01) ,C12N 15/81 (2006.01) ,C12N 9/96 (2006.01) ,C12N 9/42 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
76
Albumins
765
Serum albumin, e.g. HSA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
81
for yeasts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
96
Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
42
acting on beta-1, 4-glucosidic bonds, e.g. cellulase
Applicants:
广州中国科学院先进技术研究所 GUANGZHOU INSTITUTE OF ADVANCED TECHNOLOGY, CHINESE ACADEMY OF SCIENCES [CN/CN]; 中国广东省广州市 南沙区海滨路1121号 No.1121 Haibin Rd, Nansha District Guangzhou, Guangdong 510000, CN
Inventors:
崔金明 CUI, Jinming; CN
李小明 LI, Xiaoming; CN
张齐 ZHANG, Qi; CN
刘陈立 LIU, Chenli; CN
蒙海林 MENG, Hailin; CN
黄建东 HUANG, Jiandong; CN
Agent:
广州容大专利代理事务所(普通合伙) GUANGZHOU RONDA PATENT AGENCY; 中国广东省广州市 越秀区东风中路300号之一金安大厦14楼B室 Room B, Floor 14, JinAn Building No. 300, Dong Feng Zhong Road, Yuexiu District Guangzhou, Guangdong 510030, CN
Priority Data:
201710883353.426.09.2017CN
Title (EN) RECOMBINANT BOVINE SERUM ALBUMIN MATURE PEPTIDE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
(FR) PEPTIDE MATURE D'ALBUMINE DE SÉRUM BOVIN RECOMBINANTE, SON PROCÉDÉ DE PRÉPARATION, ET APPLICATION CORRESPONDANTE
(ZH) 一种重组牛血清蛋白成熟肽及其制备方法和应用
Abstract:
(EN) A recombinant bovine serum albumin mature peptide, having an amino acid residue sequence as shown in SEQ ID NO: 1, and an optimized nucleotide sequence encoding the recombinant bovine serum albumin mature peptide as shown in SEQ ID NO: 3. A preparation method for the recombinant bovine serum albumin mature peptide comprises: synthesizing an optimized gene of a bovine serum albumin mature peptide; then integrating the gene into an expression vector, and using the vector to transform various host cells for expression; culturing the transformed host cells to obtain a fermentation broth containing a large amount of a recombinant protein; and further separating and purifying the fermentation broth to give the product. Under mass spectrometry the above recombinant bovine serum albumin is completely consistent with a natural bovine serum albumin mature peptide sequence, has the advantage of being free from animal virus contamination and has high purity, is a natural bovine serum albumin substitute, and can be applied in biochemical pharmaceuticals and other industries.
(FR) La présente invention concerne un peptide mature d'albumine de sérum bovin recombinante, ayant une séquence de résidus d'acides aminés telle que présentée dans SEQ ID NO : 1, et une séquence nucléotidique optimisée codant pour le peptide mature d'albumine de sérum bovin recombinante telle que présentée dans SEQ ID NO : 3. Un procédé de préparation du peptide mature d'albumine de sérum bovin recombinante comprend : la synthèse d'un gène optimisé d'un peptide mature d'albumine de sérum bovin; puis l'intégration du gène dans un vecteur d'expression, et l'utilisation du vecteur pour transformer diverses cellules hôtes en vue de leur expression; la culture des cellules hôtes transformées pour obtenir un bouillon de fermentation contenant une grande quantité d'une protéine recombinante; et la séparation et la purification supplémentaires du bouillon de fermentation pour obtenir le produit. En spectrométrie de masse, l'albumine de sérum bovin recombinante ci-dessus est complètement cohérente avec une séquence de peptide mature d'albumine de sérum bovin naturelle, a l'avantage d'être exempte de contamination virale animale et présente une pureté élevée, est un substitut de l'albumine de sérum bovin naturelle, et peut être appliquée dans l'industrie des produits pharmaceutiques biochimiques et dans d'autres industries.
(ZH) 一种重组牛血清蛋白成熟肽,其氨基酸残基序列如SEQ IID NO:1所示,编码所述重组牛血清蛋白成熟肽的优化核苷酸序列如SEQ IID NO:3所示;所述重组牛血清蛋白成熟肽制备方法包括:人工合成牛血清蛋白成熟肽的优化基因;然后将该基因整合到表达载体中,并用该载体转化各种宿主细胞进行表达;通过培养已转化的宿主细胞,得到的发酵液中含有大量的重组蛋白;再对发酵液进行分离纯化即得。上述重组牛血清蛋白经质谱检测与天然牛血清蛋白成熟肽序列完全一致,具有免受动物病毒污染,纯度高等优点,是天然牛血清蛋白替代品,可以应用在生化医药等行业中。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)