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1. (WO2019062222) METHOD FOR ENZYMATIC PREPARATION OF R-3-AMINOBUTYRIC ACID
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Pub. No.: WO/2019/062222 International Application No.: PCT/CN2018/092010
Publication Date: 04.04.2019 International Filing Date: 20.06.2018
IPC:
C12P 13/04 (2006.01) ,C12N 1/19 (2006.01) ,C12N 9/88 (2006.01) ,C07K 14/245 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
24
from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
245
Escherichia (G)
Applicants:
上海弈柯莱生物医药科技有限公司 ABIOCHEM BIOTECHNOLOGY CO., LTD. [CN/CN]; 中国上海市 浦东新区中国(上海)自由贸易试验区芳春路400号1幢4层436室 Room 436, Building 1, No. 400, Fangchun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area Shanghai 201203, CN
Inventors:
孙传民 SUN, Chuanmin; CN
程占冰 CHENG, Zhanbing; CN
焦江华 JIAO, Jianghua; CN
丁少南 DING, Shaonan; CN
田振华 TIAN, Zhenhua; CN
Agent:
上海一平知识产权代理有限公司 XU & PARTNERS, LLC.; 中国上海市 普陀区真北路958号天地科技广场1号楼106室 Room 106, Building No. 1, Universal High-Tech Plaza 958 Zhen Bei Road, Putuo District Shanghai 200333, CN
Priority Data:
201710906645.529.09.2017CN
Title (EN) METHOD FOR ENZYMATIC PREPARATION OF R-3-AMINOBUTYRIC ACID
(FR) PROCÉDÉ POUR LA PRÉPARATION ENZYMATIQUE D'ACIDE R-3-AMINOBUTYRIQUE
(ZH) 酶法制备R-3-氨基丁酸的方法
Abstract:
(EN) An R-3-aminobutyric acid preparation method with high efficiency and high stereoselectivity. The method comprises using aspartase with stereoisomeric catalytic activity derived from Escherichia coli to efficiently convert butenoic acid into R-3-aminobutyric acid. After only 24 h of reaction, the conversion rate is as high as ≥ 98%, and the ee value is ≥ 99.9%. The conversion efficiency is greatly improved, the reaction time is shortened, and the production costs are reduced. The method features a high yield, a high conversion rate, low costs, a short production cycle, a simple process, ease of enlargement, suitability for mass production and the like.
(FR) L'invention concerne un procédé de préparation d'acide R-3-aminobutyrique doté d'une efficacité élevée et d'une stéréosélectivité élevée. Le procédé comprend l'utilisation d'aspartase pourvue d'une activité catalytique stéréo-isomérique dérivée d'Escherichia coli pour convertir efficacement l'acide buténoïque en acide R-3-aminobutyrique. Après seulement 24 h de réaction, le taux de conversion est aussi élevé que ≥ 98 % et la valeur d'ee est ≥ 99,9 %. L'efficacité de conversion est considérablement améliorée, le temps de réaction est raccourci et les coûts de production sont réduits. Le procédé présente un rendement élevé, un taux de conversion élevé, de faibles coûts, un cycle de production court, un processus simple, une facilité d'agrandissement, un caractère approprié pour la production de masse et similaires.
(ZH) 一种高效、高立体选择性的R-3-氨基丁酸制备方法,该方法利用来自大肠杆菌、具有立体异构催化活性的天冬氨酸酶,将丁烯酸高效地转化为R-3-氨基丁酸,反应仅24h后转化率高达≥98%,ee值≥99.9%,极大地提高转化效率,缩短反应时间,降低生产成本。所述方法具有收率高、转化率高、成本低、生产周期短、工艺简单、易于放大、适合进行大生产等诸多优点。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)