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1. (WO2019061560) IN VITRO MATURATION CULTURE MEDIUM OF IMMATURE OOCYTES AND USE THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/061560 International Application No.: PCT/CN2017/106067
Publication Date: 04.04.2019 International Filing Date: 13.10.2017
IPC:
C12N 5/075 (2010.01) ,C12N 5/071 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
075
Oocytes; Oogonia
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
Applicants:
中国农业大学 CHINA AGRICULTURAL UNIVERSITY [CN/CN]; 中国北京市 海淀区圆明园西路2号 2 Yuanmingyuan Xilu, Haidian District Beijing 100193, CN
Inventors:
夏国良 XIA, Guoliang; CN
王超 WANG, Chao; CN
王华荣 WANG, Huarong; CN
蔡晗 CAI, Han; CN
Agent:
北京集佳知识产权代理有限公司 UNITALEN ATTORNEYS AT LAW; 中国北京市 朝阳区建国门外大街22号赛特广场7层 7th Floor, Scitech Place No.22, Jian Guo Men Wai Ave., Chao Yang District Beijing 100004, CN
Priority Data:
201710920296.230.09.2017CN
Title (EN) IN VITRO MATURATION CULTURE MEDIUM OF IMMATURE OOCYTES AND USE THEREOF
(FR) MILIEU DE CULTURE DE MATURATION IN VITRO D’OVOCYTES IMMATURES ET SON UTILISATION
(ZH) 未成熟卵母细胞的体外成熟培养液及其应用
Abstract:
(EN) Provided is a method for culturing immature oocytes. The method can promote in vitro maturation of the immature oocytes, and specifically comprises using follicular cells and a culture medium for culturing same. The culture medium for culturing the follicular cells contains CNP or variants thereof or analogues thereof and an HDAC (histone deacetylase) inhibitor. Also provided are the in vitro maturation culture medium containing CNP or variants thereof or analogues thereof and the HDAC inhibitor, and related compositions thereof, and the use of the above medium, culture medium and compositions in the promotion of in vitro maturation of the immature oocytes.
(FR) La présente invention concerne un procédé de culture d’ovocytes immatures. Le procédé peut favoriser la maturation in vitro des ovocytes immatures, et comprend spécifiquement l’utilisation de cellules folliculaires et un milieu de culture pour les cultiver. Le milieu de culture pour cultiver les cellules folliculaires contient du CNP ou ses variants ou ses analogues et un inhibiteur de HDAC (histone désacétylase). La présente invention concerne également un milieu de culture de maturation in vitro contenant du CNP ou ses variants ou ses analogues et l’inhibiteur de HDAC, et ses compositions associées, et l’utilisation du milieu, du milieu de culture et des compositions ci-dessus dans la promotion de la maturation in vitro des ovocytes immatures.
(ZH) 提供了一种未成熟卵母细胞的培养方法,其可促进未成熟卵母细胞的体外成熟,具体其包含卵泡颗粒细胞和培养该卵泡颗粒细胞的培养液,所述培养该卵泡颗粒细胞的培养液中含有CNP或其变体或其类似物和HDAC(组蛋白去乙酰化酶)抑制剂。同时提供了包含CNP或其变体或其类似物和HDAC抑制剂的体外成熟培养液及其相关组合物,以及上述培养基、培养液以及组合物在促进未成熟卵母细胞体外成熟的应用。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)