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1. (WO2019061198) KIT FOR AMPLIFYING TCR FULL-LENGTH SEQUENCE AND USE THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/061198 International Application No.: PCT/CN2017/104109
Publication Date: 04.04.2019 International Filing Date: 28.09.2017
IPC:
C12Q 1/68 (2018.01) ,C12N 15/10 (2006.01) ,C12N 15/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
Applicants:
深圳华大生命科学研究院 BGI SHENZHEN [CN/CN]; 中国广东省深圳市 盐田区北山工业区综合楼 Main Building, Beishan Industrial Zone, Yantian District Shenzhen, Guangdong 518083, CN
Inventors:
周清 ZHOU, Qing; CN
王飞 WANG, Fei; CN
赵小莹 ZHAO, Xiaoying; CN
尹悦露 YIN, Yuelu; CN
李贵波 LI, Guibo; CN
赵正琦 CHAO, Cheng-chi; CN
杨乃波 YANG, Naibo; CN
Agent:
北京品源专利代理有限公司 BEYOND ATTORNEYS AT LAW; 中国北京市 海淀区莲花池东路39号西金大厦6层 F6, Xijin Centre 39 Lianhuachi East Rd. , Haidian District Beijing 100036, CN
Priority Data:
Title (EN) KIT FOR AMPLIFYING TCR FULL-LENGTH SEQUENCE AND USE THEREOF
(FR) KIT POUR L'AMPLIFICATION D'UNE SÉQUENCE DE PLEINE LONGUEUR DE TCR ET SON UTILISATION
(ZH) 一种扩增TCR全长序列的试剂盒及其应用
Abstract:
(EN) Provided in the present invention are a kit for amplifying a TCR full-length sequence and a use thereof, wherein the kit includes a nanoliter microporous chip. In the present invention, the TCR α/β matching and amplification of 5,124 single T cells at most can be carried out in one step by using the nanoliter microporous chip as a reaction vessel.
(FR) La présente invention concerne un kit pour l'amplification d'une séquence de pleine longueur de TCR et une utilisation de celui-ci, le kit comprenant une puce microporeuse à l'échelle du nanolitre. Dans la présente invention, l'appariement et l'amplification de TCR α/β de 5124 lyphocytes T individuels au maximum peuvent être effectués en une seule étape à l'aide de la puce microporeuse à l'échelle du nanolitre en tant que cuve de réaction.
(ZH) 本发明提供了一种扩增TCR全长序列的试剂盒及其应用,所述试剂盒包含纳升级微孔芯片。本发明通过采用纳升级微孔芯片作为反应容器,一次最多可进行5124个单T细胞的TCRα/β配对扩增。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)