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1. WO2019060907 - HIGH EFFICIENCY TARGETED IN SITU GENOME-WIDE PROFILING

Publication Number WO/2019/060907
Publication Date 28.03.2019
International Application No. PCT/US2018/052707
International Filing Date 25.09.2018
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6804 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6804Nucleic acid analysis using immunogens
C40B 40/06 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
04Libraries containing only organic compounds
06Libraries containing nucleotides or polynucleotides, or derivatives thereof
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
CPC
C12Q 1/6804
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6804Nucleic acid analysis using immunogens
G01N 33/6872
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
Applicants
  • FRED HUTCHINSON CANCER RESEARCH CENTER [US]/[US]
Inventors
  • HENIKOFF, Steven
  • KAYA OKUR, Hatice, Seda
  • BRYSON, Terri, Dawn
  • SKENE, Peter, James
Agents
  • GRISWOLD, Ian, J.
  • BERNADICOU, Michael, A.
  • BLAIR, Steven, R.
  • AUYEUNG, Aloysius, T.c.
  • ABDULLAHI, Abdullahi, S.
Priority Data
62/562,91825.09.2017US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) HIGH EFFICIENCY TARGETED IN SITU GENOME-WIDE PROFILING
(FR) PROFILAGE PANGÉNOMIQUE IN SITU CIBLÉ DE HAUTE EFFICACITÉ
Abstract
(EN)
A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell, including: contacting a permeabilized cell or nucleus with a specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the specific binding agent is linked to a nuclease that is inactive or an activatable transposome; activating the nuclease or transposase, thereby excising the sequence of chromatin DNA bound to the chromatin- associated factor of interest; isolating the excised DNA; and determining the sequence of the excised DNA, thereby detecting binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in the cell.
(FR)
L'invention concerne un procédé pour la détection de la liaison d'un facteur d'intérêt associé à la chromatine à une séquence d'ADN de chromatine dans une cellule, comprenant : la mise en contact d'une cellule ou d'un noyau perméabilisés avec un agent de liaison spécifique qui reconnaît spécifiquement le facteur d'intérêt associé à la chromatine, l'agent de liaison spécifique étant lié à une nucléase qui est inactive ou à un transposome activable ; l'activation de la nucléase ou de la transposase, ce qui permet d'exciser la séquence d'ADN de chromatine lié au facteur d'intérêt associé à la chromatine ; l'isolement de l'ADN excisé ; et la détermination de la séquence de l'ADN excisé, ce qui permet de détecter la liaison d'un facteur d'intérêt associé à la chromatine à une séquence d'ADN de chromatine dans la cellule.
Latest bibliographic data on file with the International Bureau