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1. WO2019059645 - DNA APTAMER BINDING SPECIFICALLY TO TB7.7 AND USE THEREOF

Publication Number WO/2019/059645
Publication Date 28.03.2019
International Application No. PCT/KR2018/011067
International Filing Date 19.09.2018
IPC
C12N 15/115 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith
G01N 33/553 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
551the carrier being inorganic
553Metal or metal coated
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
CPC
C12N 15/115
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
C12N 2310/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
10Type of nucleic acid
16Aptamers
G01N 33/553
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
551the carrier being inorganic
553Metal or metal coated
G01N 33/5695
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56911Bacteria
5695Mycobacteria
Applicants
  • 주식회사 엠디엡투스 MD APTUS INC. [KR]/[KR]
Inventors
  • 김윤근 KIM, Yoon-Keun
  • 반창일 BAN, Changill
  • 장철훈 CHANG, Chulhun
Agents
  • 이명진 LEE, Myoung-Jin
Priority Data
10-2017-012338525.09.2017KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) DNA APTAMER BINDING SPECIFICALLY TO TB7.7 AND USE THEREOF
(FR) APTAMÈRE D'ADN SE LIANT SPÉCIFIQUEMENT À TB7.7, ET SON UTILISATION
(KO) TB7.7에 특이적으로 결합하는 DNA 압타머 및 이의 용도
Abstract
(EN)
The present invention relates to a DNA aptamer binding specifically to tuberculosis specific antigen 7.7 kDa (TB7.7), a biosensor for diagnosis of tuberculosis, comprising the same, and a method for providing information for diagnosis of tuberculosis. The present inventors found that not only does a DNA aptamer according to the present invention have specific binding potential to TB7.7 protein, but also the binding affinity is excellent. When used, the DNA aptamer of the present invention can be thus expected to exhibit greater stability than a conventional ELISA method using an antibody. Hence, the aptamer is expected to find useful applications in the development of compositions for tuberculosis diagnosis, biosensors for tuberculosis diagnosis, and information providing methods for tuberculosis diagnosis.
(FR)
La présente invention concerne un aptamère d'ADN se liant spécifiquement à l'antigène spécifique de la tuberculose 7.7 kDa (TB7.7), un biocapteur destiné au diagnostic de la tuberculose, comprenant ledit aptamère, et un procédé destiné à apporter des informations, destiné au diagnostic de la tuberculose. Selon la présente invention, il a été découvert que non seulement un aptamère d'ADN possède un potentiel de liaison spécifique à la protéine TB7.7, mais également que l'affinité de liaison est excellente. Selon la présente invention, lors de son utilisation, l'aptamère d'ADN devrait donc présenter une plus grande stabilité qu'un procédé ELISA classique utilisant un anticorps. Par conséquent, l'aptamère devrait trouver des applications avantageuses dans le développement de compositions destinées au diagnostic de la tuberculose, de biocapteurs destinés au diagnostic de la tuberculose et de procédés d'apport d'informations destinés au diagnostic de la tuberculose.
(KO)
본 발명은 TB7.7(Tuberculosis specific antigen 7.7 kDa)에 특이적으로 결합하는 DNA 압타머, 이를 포함하는 결핵 진단용 바이오센서, 및 결핵 진단을 위한 정보제공 방법에 관한 것으로, 본 발명자들은 본 발명에 따른 DNA 압타머(DNA Aptamer)가 TB7.7 단백질에 대하여 특이적인 결합능을 가질 뿐만이 아니라, 그 결합력 또한 우수하다는 사실을 확인하였다. 따라서 본 발명의 DNA 압타머를 이용한다면, 기존의 항체를 이용한 ELISA 방법보다 뛰어난 안정성을 기대할 수 있어, 상기 압타머는 결핵 진단용 조성물, 결핵 진단용 바이오센서, 결핵 진단을 위한 정보제공 방법 등의 개발 시에 유용하게 이용될 수 있을 것으로 기대된다.
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