Processing

Please wait...

Settings

Settings

Goto Application

1. WO2019059337 - NOOTKATONE PRODUCTION METHOD

Publication Number WO/2019/059337
Publication Date 28.03.2019
International Application No. PCT/JP2018/034996
International Filing Date 21.09.2018
IPC
C12P 7/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
C07C 49/637 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
49Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
587Unsaturated compounds containing a keto group being part of a ring
613polycyclic
617a keto group being part of a condensed ring system
623having two rings
637the condensed ring system containing ten carbon atoms
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 9/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
04acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C12N 15/53 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
CPC
C07C 49/637
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
49Ketones; Ketenes; Dimeric ketenes
587Unsaturated compounds containing a keto groups being part of a ring
613polycyclic
617a keto group being part of a condensed ring system
623having two rings
637the condensed ring system containing ten carbon atoms
C12P 7/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
Applicants
  • 味の素株式会社 AJINOMOTO CO., INC. [JP]/[JP]
Inventors
  • 田島 義教 TAJIMA, Yoshinori
Agents
  • 特許業務法人酒井国際特許事務所 SAKAI INTERNATIONAL PATENT OFFICE
Priority Data
2017-18244822.09.2017JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) NOOTKATONE PRODUCTION METHOD
(FR) PROCÉDÉ DE FABRICATION DE NOOTKATONE
(JA) ヌートカトンの製造方法
Abstract
(EN)
The present invention provides an efficient method for producing nootkatone via a biological method. More specifically, the present invention provides a nootkatone production method including converting nootkatole to nootkatone in the presence of (i) a transformant microorganism having improved alcohol dehydrogenase activity over a wild-type organism, or (ii) alcohol dehydrogenase, the alcohol dehydrogenase being: (A) a protein having the amino acid sequence of SEQ ID NO. 30 or 32; (B) a protein having the amino acid sequence of SEQ ID NO. 30 or 32 with one or more amino acid substitutions, deletions, insertions, or additions, and having alcohol dehydrogenase activity; or (C) a protein having an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO. 30 or 32, and having alcohol dehydrogenase activity.
(FR)
L'invention fournit un procédé de fabrication efficace de nootkatone selon un procédé biologique. Plus précisément, l'invention fournit un procédé de fabrication de nootkatone qui inclut une étape au cours de laquelle un nootkatol est converti en nootkatone en présence (i) d'un microorganisme transformé présentant un alcool déshydrogénase amélioré en comparaison avec un microorganisme sauvage, (ii) et d'un alcool déshydrogénase. L'alcool déshydrogénase consiste en : (A) une protéine contenant une séquence d'acides aminés de SEQ ID No 30 ou 32 ; (B) une protéine qui contient une séquence d'acides aminés incluant la substitution, la délétion, l'insertion ou l'addition d'un ou plusieurs acides aminés, dans la séquence d'acides aminés de SEQ ID No 30 ou 32, et qui possède une activité d'alcool déshydrogénase ; ou (C) une protéine qui contient une séquence d'acides aminés possédant 90% d'identité ou plus avec la séquence d'acides aminés de SEQ ID No 30 ou 32, et qui possède une activité d'alcool déshydrogénase.
(JA)
本発明は、生物学的方法によるヌートカトンの効率的な製造方法を提供する。より具体的には、本発明は、(i)アルコールデヒドロゲナーゼの活性が野生型微生物に比し向上した形質転換微生物、または(ii)アルコールデヒドロゲナーゼの存在下において、ヌートカトールをヌートカトンに変換することを含み、 アルコールデヒドロゲナーゼが、下記: (A)配列番号30または32のアミノ酸配列を含むタンパク質; (B)配列番号30または32のアミノ酸配列において、1もしくは数個のアミノ酸の置換、欠失、挿入、もしくは付加を含むアミノ酸配列を含み、かつ、アルコールデヒドロゲナーゼ活性を有するタンパク質;または (C)配列番号30または32のアミノ酸配列に対して90%以上の同一性を有するアミノ酸配列を含み、かつ、アルコールデヒドロゲナーゼ活性を有するタンパク質である、ヌートカトンの製造方法を提供する。
Latest bibliographic data on file with the International Bureau