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1. WO2019056205 - TMV RESISTANT TOBACCO PLANT CONTAINING SHORT N INTRODUCED FRAGMENT AND METHOD FOR BREEDING SAME

Publication Number WO/2019/056205
Publication Date 28.03.2019
International Application No. PCT/CN2017/102444
International Filing Date 20.09.2017
IPC
A01H 5/00 2018.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
A01H 1/02 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
02Methods or apparatus for hybridisation; Artificial pollination
C12Q 1/68 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12N 15/11 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
CPC
A01H 1/02
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
02Methods or apparatus for hybridisation; Artificial pollination
A01H 5/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
Applicants
  • 云南省烟草农业科学研究院 YUNNAN ACADEMY OF TOBACCO AGRICULTURAL SCIENCES [CN]/[CN]
Inventors
  • 刘勇 LIU, Yong
  • 黄昌军 HUANG, Changjun
  • 李永平 LI, Yongping
  • 于海芹 YU, Haiqin
Agents
  • 北京柏杉松知识产权代理事务所(普通合伙) PATENTSINO IP FIRM
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) TMV RESISTANT TOBACCO PLANT CONTAINING SHORT N INTRODUCED FRAGMENT AND METHOD FOR BREEDING SAME
(FR) PLANT DE TABAC RÉSISTANT AU TMV CONTENANT UN FRAGMENT COURT N INTRODUIT ET SON PROCÉDÉ DE SÉLECTION
(ZH) 包含短N导入片段的抗TMV的烟草植株及其选育方法
Abstract
(EN)
A TMV resistant tobacco plant containing a short N introduced fragment and a method for breeding the same. A homozygous tobacco plant containing an N introduced fragment is hybridized with a tobacco plant having a genotype of nn, to obtain an F1 progeny tobacco plant having a genotype of Nn. The F1 progeny tobacco plant is hybridized with the tobacco plant having the genotype of nn, to obtain population materials for screening to obtain the short N introduced fragment. TMV is inoculated at a seedling stage, and Nn genotype plants showing plaques are obtained by screening the population materials. The Nn genotype plants are genotyped using a molecular marker TN5.51 primer pair and an N gene-specific molecular marker N1N2 at the right end of the N introduced fragment. A plant found to be negative when tested by the TN5.51 primer pair and to be positive when tested by the N1N2 molecular marker is a plant containing the short N introduced fragment. The number of non-target gene components deleted from plants containing the short N introduced fragment is estimated using TN5.34 and TN5.20 and TN4.99 primer pairs. The obtained short N introduced fragment is applicable to resource formulation and selective breeding of TMV resistant tobacco. The invention is helps to reduce cumbersome linkage with an N gene.
(FR)
L'invention concerne un plant de tabac résistant au TMV contenant un fragment court N introduit, et son procédé de sélection. Un plant de tabac homozygote contenant un fragment N introduit est hybridé avec un plant de tabac ayant un génotype nn, afin d'obtenir un plant de tabac descendant F1 ayant un génotype Nn. Le plant de tabac descendant F1 est hybridé avec le plant de tabac ayant le génotype nn, afin d'obtenir des matériels de population à cribler en vue d'obtenir le fragment N court introduit. Le TMV est inoculé au niveau du stade de semis, et des plants de génotype Nn présentant des plaques sont obtenus par criblage des matériels de population. Les plants de génotype Nn sont génotypés à l'aide d'une paire d'amorces TN5.51 de marquage moléculaire et d'un marqueur moléculaire N1N2 spécifique du gène N à l'extrémité droite du fragment N introduit. Un plant qui se révèle négatif lorsqu'il est analysé au moyen de la paire d'amorces TN5.51 et positif lorsqu'il est analysé au moyen du marqueur moléculaire N1N2 est un plant contenant le fragment court N introduit. Le nombre de composants de gènes non cibles délétés dans les plants contenant le fragment court N introduit est estimé à l'aide des paires d'amorces TN5.34, TN5.20 et TN4.99. Le fragment court N introduit obtenu est applicable à la formulation de ressources et à la reproduction sélective de tabac résistant au TMV. L'invention permet de réduire la liaison encombrante avec un gène N.
(ZH)
包含短N导入片段的抗TMV的烟草植株及其选育方法,利用N导入片段纯合的烟草与基因型为nn的烟草杂交,获得基因型为Nn的F1子代烟草。利用F1子代烟草与基因型为nn的烟草杂交,获得用于筛选短N导入片段的群体材料。苗期接种TMV,在群体材料中筛选出表现枯斑的Nn基因型植株。采用N导入片段右末端的分子标记TN5.51引物对和N基因特异分子标记N1N2对Nn基因型植株进行基因型分型,TN5.51引物对检测为阴性且N1N2分子标记为阳性的植株,就是短N导入片段的植株。利用TN5.34和TN5.20和TN4.99引物对估算短N导入片段的植株缺失的非目的基因组分数量。获得的短N导入片段的植株可应用于烟草TMV抗病资源创制和品种选育,有利于减少与N基因连锁的累赘。
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