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1. WO2019056020 - NANOCELLULOSE AND NANOCELLULOSE COMPOSITE ARRAYS AND DEVICES AND METHODS OF MAKING AND USING THE SAME

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CLAIMS

What is claimed is:

1. A microneedle array comprising a plurality of microneedles comprising nanocellulose or a nanocellulose composite.

2. The microneedle array of claim 1 , wherein the nanocellulose or nanocellulose composite comprises crystalline nanocellulose, semi-crystalline nanocellulose, cellulose nanofibers, microfibrillated cellulose, bacterial nanocellulose, wood-based nanocellulose, or a combination thereof.

3. The microneedle array of claim 1 or claim 2, wherein the plurality of microneedles comprise a nanocellulose composite, and the nanocellulose composite comprises hyaluronic acid, poly(lactic-co-glycolic acid), poly(ethylene glycol), gelatin, alginate, chitosan, (2,2,6,6-tetramethyl-piperidin-l -yl)oxyl oxidized nanocellulose, or a combination thereof.

4. The microneedle array of any one of claims 1-3, wherein at least one of the plurality of microneedles is a hollow microneedle.

5. The microneedle array of any one of claims 1-4, wherein at least one of the plurality of microneedles has a diameter from about 100 μm to about 1000 μm.

6. The microneedle array of any one of claims 1-5, wherein at least one of the plurality of microneedles has a tip width of less than or equal to about 10 μm.

7. The microneedle array of any one of claims 1-6, wherein at least one of the plurality of microneedles is pyramidal with a base dimension of about 250 μm by about 250 μm and a height of about 500 μm.

8. The microneedle array of any one of claims 1-7, wherein the nanocellulose or

nanocellulose composite has a fibril density from about 0.01 to about 0.9 g/mL.

9. The microneedle array of any one of claims 1-8, wherein the nanocellulose or

nanocellulose composite has a fibril thickness of from about 10 nm to about 1 μm.

10. The microneedle array of any one of claims 1-9, wherein the plurality of microneedles can withstand a compressive load of at least about 15 N without breaking.

11. A method of producing a microneedle array comprising:

a) casting a microneedle array by applying a nanocellulose or nanocellulose composite slurry to a microneedle mold; and

b) extracting the microneedle array from the microneedle mold.

12. The method of claim 11, further comprising attaching the microneedle array to a stretchable backing film.

13. A device comprising:

a) a microneedle array comprising a plurality of microneedles comprising nanocellulose or a nanocellulose composite; and

b) a sorbent film connected to the microneedle array.

14. The device of claim 13, wherein the nanocellulose or nanocellulose composite comprises crystalline nanocellulose, semi-crystalline nanocellulose, cellulose nanofibers, microfibrillated cellulose, bacterial nanocellulose, wood-based nanocellulose, or a combination thereof.

15. The device of claim 13 or claim 14, wherein the sorbent film comprises hyaluronic acid, polyvinyl alcohol, polyethylene glycol, a biocompatible sorbent, or a combination thereof.

16. The device of any one of claims 13-1 5, further comprising a biosensor for detecting or measuring an analyte from a fluid.

17. The device of claim 16, wherein the device is capable of detecting or measuring the analyte continuously for at least a duration of time greater than about 10 min without mechanical or chemical pumping.

18. A method of extracting a dermal biofluid or subdermal biofluid from a living subject having a stratum corneum, the method comprising:

a) applying a microneedle array comprising nanocellulose or a nanocellulose composite to a region of skin of the living subject;

b) penetrating the stratum corneum of skin with the microneedle array; and

c) extracting a dermal biofluid or subdermal biofluid from the living subject with the microneedle array without mechanical or chemical pumping.

19. The method of claim 18, further comprising analyzing the biofluid with an immunoassay.

20. The method of claim 18 or claim 19, further comprising continuously extracting a subcutaneous fluid from the living subject for at least a duration of time greater than about 10 min.

21. The microneedle array of any one of claims 1-6, wherein at least one of the plurality of microneedles is conical.

22. The method of clam 11, further comprising:

al) drying the cast microneedle array of a) under controlled temperature and humidity; and

a2) in situ crosslinking the microneedle array of al) before the extraction of b).

23. The device of any one of claims 13-17, wherein the sorbent film connected to the microneedle array controls the flow of fluid through the microneedle array via passive sample collection and capillary force.