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1. WO2019056002 - CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS

Publication Number WO/2019/056002
Publication Date 21.03.2019
International Application No. PCT/US2018/051557
International Filing Date 18.09.2018
IPC
C12N 15/01 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C12N 15/10 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/64 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C12N 15/70 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
CPC
C12N 15/1055
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1055Protein x Protein interaction, e.g. two hybrid selection
C12N 15/1058
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
C12N 15/111
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
111General methods applicable to biologically active non-coding nucleic acids
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
Applicants
  • PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US]
Inventors
  • LIU, David, R.
  • BADRAN, Ahmed, Hussein
  • WANG, Tina
Agents
  • MACDONALD, Kevin
Priority Data
62/559,91918.09.2017US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS
(FR) ÉVOLUTION CONTINUE POUR PROTÉINES STABILISÉES
Abstract
(EN)
Some aspects of this disclosure relate to systems, apparatuses, compositions (e.g., isolated nucleic acids and vectors), and methods for improving the stability and/or solubility of proteins evolved using phage-assisted continuous evolution (PACE). In some embodiments, vectors described herein comprise nucleic acids encoding selection systems (e.g., positive and/or negative selection systems) that link expression of genes required for production of infectious phage particles to a desirable physiochemical (e.g., stability or solubility) and/or desired function of an evolved protein.
(FR)
Certains aspects de la présente invention concernent des systèmes, des appareils, des compositions (par exemple, des acides nucléiques isolés et des vecteurs), et des procédés d'amélioration de la stabilité et/ou de la solubilité de protéines évoluées obtenues par évolution continue assistée par phages (PACE). Dans certains modes de réalisation, les vecteurs décrits ici comprennent des acides nucléiques codant des systèmes de sélection (par exemple, des systèmes de sélection positive et/ou négative) qui relient l'expression de gènes requis pour la production de particules de phage infectieuses à une caractéristique physicochimique souhaitable (par exemple, stabilité ou solubilité) et/ou une fonction souhaitée d'une protéine évoluée.
Also published as
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