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1. WO2019055985 - CLICK CHEMISTRY APTAMER TAGGING FOR EAB BIOSENSORS

Publication Number WO/2019/055985
Publication Date 21.03.2019
International Application No. PCT/US2018/051516
International Filing Date 18.09.2018
IPC
A61B 5/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Measuring for diagnostic purposes; Identification of persons
A61B 5/05 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Measuring for diagnostic purposes; Identification of persons
05Measuring for diagnosis by means of electric currents or magnetic fields
A61B 5/053 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Measuring for diagnostic purposes; Identification of persons
05Measuring for diagnosis by means of electric currents or magnetic fields
053Measuring electrical impedance or conductance of a portion of the body
A61B 10/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
10Other methods or instruments for diagnosis, e.g. for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC
A61B 10/0064
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
10Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis
0045Devices for taking samples of body liquids
0064for taking sweat or sebum samples
A61B 2562/12
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
2562Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
12Manufacturing methods specially adapted for producing sensors for in-vivo measurements
A61B 5/0538
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
05Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; ; Measuring using microwaves or radiowaves
053Measuring electrical impedance or conductance of a portion of the body
0538invasively, e.g. using a catheter
A61B 5/14735
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
145Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
1468using chemical or electrochemical methods, e.g. by polarographic means
1473invasive, e.g. introduced into the body by a catheter
14735comprising an immobilised reagent
C12N 15/115
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
C12N 2310/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
10Type of nucleic acid
16Aptamers
Applicants
  • ECCRINE SYSTEMS, INC. [US]/[US]
Inventors
  • BERTRAND, Jacob, A.
  • HANLEY, Brian
  • SAFAZADEH, Haghighi, Leila
Agents
  • CLARK, Chad, G.
Priority Data
62/559,85718.09.2017US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CLICK CHEMISTRY APTAMER TAGGING FOR EAB BIOSENSORS
(FR) MARQUAGE D'APTAMÈRES DE CHIMIE CLICK POUR BIOCAPTEURS EAB
Abstract
(EN)
Methods are disclosed for using click chemistry techniques to functionalize aptamer sequences for use in electrochemical aptamer-based biosensors, and in particular such sensors for use in sweat sensing environments. Specifically, such techniques include preparing a portion of the aptamer sequence to interact with a functionalization component through a bio-orthogonal chemistry reaction, preparing the functionalization component to interact with the aptamer sequence through the click chemistry reaction, and attaching the aptamer sequence to the functionalization component through the click chemistry reaction. The functionalization component may be one or more of an oligonucleotide primer, an anchor molecule, a redox moiety, an oligonucleotide dock, or an electrode surface. Disclosed bio-orthogonal chemistry reactions include an azide-alkyne cycloaddition reaction, and a thiol-maleimide reaction.
(FR)
L'invention concerne des procédés d'utilisation de techniques de chimie click pour fonctionnaliser des séquences d'aptamères pour une utilisation dans des biocapteurs à base d'aptamères électrochimiques, et en particulier de tels capteurs destinés à être utilisés dans des environnements de détection de transpiration. Spécifiquement, de telles techniques comprennent de préparer une partie de la séquence d'aptamères pour interagir avec un composant de fonctionnalisation par l'intermédiaire d'une réaction de chimie bio-orthogonale, préparer le composant de fonctionnalisation pour interagir avec la séquence d'aptamères par l'intermédiaire de la réaction de chimie click, et fixer la séquence d'aptamères au composant de fonctionnalisation par l'intermédiaire de la réaction de chimie click. Le composant de fonctionnalisation peut être un ou plusieurs parmi une amorce oligonucléotidique, une molécule d'ancrage, une fraction redox, un dock oligonucléotidique ou une surface d'électrode. Les réactions de chimie bio-orthogonale de l'invention comprennent une réaction de cycloaddition azide-alcyne, et une réaction thiol-maléimide.
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