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1. WO2019055535 - METHODS OF GENOMIC INTEGRATION FOR HOST CELLS INCLUDING HANSENULA AND ARXULA

Publication Number WO/2019/055535
Publication Date 21.03.2019
International Application No. PCT/US2018/050706
International Filing Date 12.09.2018
IPC
C12N 15/90 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
CPC
C12N 15/102
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
102Mutagenizing nucleic acids
C12N 15/905
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
902using homologous recombination
905in yeast
Applicants
  • AMYRIS, INC. [US]/[US]
Inventors
  • HORWITZ, Andrew
  • TAI, Anna
Agents
  • BASTIAN, Kevin L.
  • HINSCH, Matthew E.
  • YAO, Zhengzheng
  • DODDRIDGE, Zara A.
  • FERNANDEZ, Lizette
Priority Data
62/560,05718.09.2017US
62/667,02004.05.2018US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF GENOMIC INTEGRATION FOR HOST CELLS INCLUDING HANSENULA AND ARXULA
(FR) PROCÉDÉS D'INTÉGRATION GÉNOMIQUE POUR CELLULES HÔTES COMPRENANT HANSENULA ET ARXULA
Abstract
(EN) The present invention provides high efficiency targeted single, double, or multiple genomic integrations of donor nucleic acids by using CRISPR in host cells, including Hansenula and Arxula.
(FR) La présente invention concerne des intégrations génomiques simples, doubles ou multiples ciblées, de haute efficacité, d'acides nucléiques donneurs à l'aide de CRISPR dans des cellules hôtes, comprenant Hansenula et Arxula.
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