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1. WO2019055481 - METHODS FOR GENOMIC INTEGRATION IN PICHIA AND OTHER HOST CELLS

Publication Number WO/2019/055481
Publication Date 21.03.2019
International Application No. PCT/US2018/050613
International Filing Date 12.09.2018
IPC
C12N 15/90 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
CPC
C12N 15/815
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
815for yeasts other than Saccharomyces
C12N 15/905
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
902using homologous recombination
905in yeast
Applicants
  • AMYRIS, INC. [US]/[US]
Inventors
  • JIANG, Hanxiao
Agents
  • BASTIAN, Kevin L.
  • HINSCH, Matthew E.
  • YAO, Zhengzheng
  • DODDRIDGE, Zara A.
  • FERNANDEZ, Lizette
Priority Data
62/560,02618.09.2017US
62/666,92304.05.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR GENOMIC INTEGRATION IN PICHIA AND OTHER HOST CELLS
(FR) PROCÉDÉS D'INTÉGRATION GÉNOMIQUE DANS PICHIA ET AUTRES CELLULES HÔTES
Abstract
(EN)
The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
(FR)
La présente invention offre des intégrations simples, doubles, triples, quadruples et quintuples, très efficaces, ciblées et sans marqueur, à l'aide de CRISPR, dans des cellules hôtes, y compris Pichia.
Also published as
Latest bibliographic data on file with the International Bureau