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1. WO2019051852 - RECOMBINANT METARHIZIUM ACRIDUM, PREPARATION METHOD THEREFOR AND USE THEREOF

Publication Number WO/2019/051852
Publication Date 21.03.2019
International Application No. PCT/CN2017/102276
International Filing Date 19.09.2017
IPC
C12N 1/15 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
15modified by introduction of foreign genetic material
A01N 63/04 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
63Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
04Microbial fungi;  Substances produced thereby or obtained therefrom
A01P 7/04 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
7Arthropodicides
04Insecticides
C12R 1/645 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
645Fungi
CPC
A01N 63/30
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
63Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
30Microbial fungi; Substances produced thereby or obtained therefrom
C12N 9/1217
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1217Phosphotransferases with a carboxyl group as acceptor (2.7.2)
C12Y 207/02003
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
207Transferases transferring phosphorus-containing groups (2.7)
02Phosphotransferases with a carboxy group as acceptor (2.7.2)
02003Phosphoglycerate kinase (2.7.2.3)
Applicants
  • 中国科学院动物研究所 INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES [CN]/[CN]
Inventors
  • 王云丹 WANG, Yundan
  • 胡帅 HU, Shuai
  • 康乐 KANG, Le
Agents
  • 北京布瑞知识产权代理有限公司 BRIGHTHEAD INTELLECTUAL PROPERTY AGENCY CO., LTD.
Priority Data
201710830876.212.09.2017CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) RECOMBINANT METARHIZIUM ACRIDUM, PREPARATION METHOD THEREFOR AND USE THEREOF
(FR) METARHIZIUM ACRIDUM RECOMBINANT, PROCÉDÉ DE PRÉPARATION ASSOCIÉ ET UTILISATION CORRESPONDANTE
(ZH) 重组蝗绿僵菌及其制备方法和应用
Abstract
(EN)
Disclosed is a recombinant Metarhizium acridum capable of expressing phosphoglycerate kinase. The gene introduced in the recombinant Metarhizium acridum is the phosphoglycerate kinase gene, which can significantly increase the concentration of fructose-6-phosphate in the obligate bacterium, Metarhizium acridum, and significantly improve the insecticidal efficiency such that the median lethal time, LT50, of Metarhizium acridum is reduced from 7.045 ± 0.211 days to 5.617 ± 0.187 days. In addition, the recombinant Metarhizium acridum is harmless to the environment, biologically safe and non-toxic to humans.
(FR)
La présente invention concerne un Metarhizium acridum recombinant capable d'exprimer la phosphoglycérate kinase. Le gène introduit dans le Metarhizium acridum recombinant est le gène de la phosphoglycérate kinase, qui peut augmenter significativement la concentration en fructose-6-phosphate dans la bactérie obligatoire, Metarhizium acridum, et améliorer significativement l'efficacité insecticide de telle sorte que le temps létal médian, LT50, de Metarhizium acridum est réduit de 7,045 ± 0,211 jours à 5,617 ± 0,187 jours. De plus, le Metarhizium acridum recombinant est inoffensif pour l'environnement, biologiquement sûr et non toxique pour les êtres humains.
(ZH)
一种重组蝗绿僵菌,其能够表达磷酸甘油酸激酶。在该重组蝗绿僵菌中导入的基因为磷酸甘油酸激酶基因,其能够显著提高专性菌蝗绿僵菌中果糖-6-磷酸的浓度,显著提高杀虫效率,使蝗绿僵菌的半致死时间LT50从7.045±0.211天缩短到5.617±0.187天;且对环境无害,生物安全性好,对人类无毒性。
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