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1. WO2019051642 - KIT FOR TRANSFECTING INTRACELLULAR PARASITES AND USE THEREOF

Publication Number WO/2019/051642
Publication Date 21.03.2019
International Application No. PCT/CN2017/101404
International Filing Date 12.09.2017
IPC
C12N 15/87 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
CPC
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12N 15/87
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Applicants
  • 广州中科蓝华生物科技有限公司 GUANGZHOU CAS LAMVAC BIOTECH CO., LTD [CN]/[CN]
Inventors
  • 胡文 HU, Wen
  • 单雪凤 SHAN, Xuefeng
  • 姚永超 YAO, Yongchao
  • 秦莉 QIN, Li
  • 陈小平 CHEN, Xiaoping
Agents
  • 北京品源专利代理有限公司 BEYOND ATTORNEYS AT LAW
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) KIT FOR TRANSFECTING INTRACELLULAR PARASITES AND USE THEREOF
(FR) KIT POUR LA TRANSFECTION DE PARASITES INTRACELLULAIRES ET SON UTILISATION
(ZH) 一种转染细胞内寄生虫的试剂盒及其应用
Abstract
(EN)
Disclosed are a kit for transfecting intracellular parasites and the use thereof. The kit comprises polyethyleneimine, and further comprises saponin and/or polyethylene glycol octylphenyl ether. The kit transfects rapidly, has a high transfection efficiency and a high success rate of transfection, takes a short amount of time to acquire positive strains after transfection and does not require long-term drug screening, requires only a very small amount of chemical transfection reagents and does not require expensive transfection instruments, has low cost of raw materials, has a relatively low consumption of the reagents for single transfection, and has the potential to transfect multiple gene expression vectors at one time.
(FR)
L'invention concerne un kit pour la transfection de parasites intracellulaires et son utilisation. Le kit comprend de la polyéthylèneimine et comprend en outre de la saponine et/ou de l'éther octylphénylique de polyéthylèneglycol. Le kit permet une transfection rapide, il a une efficacité de transfection élevée et un taux de réussite de transfection élevé, il prend peu de temps pour acquérir des souches positives après transfection et ne nécessite pas de criblage de médicament à long terme, il ne nécessite qu'une très petite quantité de réactifs de transfection chimiques et ne nécessite pas d'instruments de transfection coûteux, il utilise des matières premières de faible coût, il a une consommation relativement faible des réactifs pour une seule transfection et il permet de transfecter de multiples vecteurs d'expression génique en une seule fois.
(ZH)
一种转染细胞内寄生虫的试剂盒及其应用,所述试剂盒包括聚乙烯亚胺,所述试剂盒还包括皂素和/或聚乙二醇辛基苯基醚。所述试剂盒转染快速,转染效率高,转染成功率高;转染后阳性虫株获取耗时短,不需要长时间药物筛选;仅需要极少量的化学转染试剂,不需要昂贵的转染仪器;原料成本低,单次转染的试剂耗费较低;具有一次转染多个基因表达载体的潜力。
Also published as
Latest bibliographic data on file with the International Bureau