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1. (WO2019050420) NOVEL METHOD FOR THE ISOLATION OF BIOGENIC AMINES FROM BIOLOGICAL MATRICES
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Pub. No.: WO/2019/050420 International Application No.: PCT/PL2018/000087
Publication Date: 14.03.2019 International Filing Date: 07.09.2018
IPC:
G01N 27/447 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/68 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
416
Systems
447
using electrophoresis
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
GDAŃSKI UNIWERSYTET MEDYCZNY [PL/PL]; PL
Inventors:
GDAŃSKI UNIWERSYTET MEDYCZNY; PL
OLĘDZKA, Ilona; PL
MIĘKUS - PURWIN, Nathalia; PL
BĄCZEK, Tomasz; PL
PLENIS, Alina; PL
KOWALSKI, Piotr; PL
Agent:
KANCELARIA PRAWNO-PATENTOWA; Małgorzata Matyka ul. Zielony Stok 12/1U PL-80-119 Gdańsk, PL
Priority Data:
P.42278808.09.2017PL
Title (EN) NOVEL METHOD FOR THE ISOLATION OF BIOGENIC AMINES FROM BIOLOGICAL MATRICES
(FR) NOUVEAU PROCÉDÉ D'ISOLEMENT D'AMINES BIOGÈNES À PARTIR DE MATRICES BIOLOGIQUES
Abstract:
(EN) The subject-matter of this invention is a method for the isolation of the biogenic amines from biological matrices, where the extraction procedure of seven biogenic amines and their precursor amino acids from the biological material comprises: • - activation of polystyrene-divinylbenzene (PS-DVB) solid phase microextraction (SPME ) coatings with a mixture of methanohwater (50:50; v/v) for 30 minutes; followed by rinsing with deionized water for 10 seconds; • - extraction of acidified biological samples, containing the tested analytes, wherein extraction time is 90 minutes; • - washing of SPME coatings with deionized water for 10 seconds; • - desorption of the analytes for 90 minutes with 2 ml_ ionic liquid- enriched methanol; • - evaporation of desorbent under a decreased pressure, at temperature of 40 °C; • - dissolution of the dry residue in 50 μΙ_ of sample buffer; • - electrophoretic analysis (by MEKC, micellar electrokinetic chromatography).
(FR) La présente invention a pour objet de pourvoir à un procédé d'isolement d'amines biogènes à partir de matrices biologiques, où la procédure d'extraction de sept amines biogènes et de leurs acides aminés précurseurs à partir du matériel biologique comprend : • - l'activation de revêtements de micro-extraction en phase solide (SPME) de polystyrène-divinylbenzène (PS-DVB) à l'aide d'un mélange méthanol-eau (50:50 ; v/v) pendant 30 minutes ; suivie d'un rinçage à l'eau désionisée pendant 10 secondes ; • - l'extraction d'échantillons biologiques acidifiés, contenant les analytes testés, où le temps d'extraction est de 90 minutes ; • - le lavage des revêtements SPME à l'eau désionisée pendant 10 secondes ; • - la désorption des analytes pendant 90 minutes avec 2 ml de méthanol enrichi en liquide ionique ; • - l'évaporation du désorbant sous pression réduite, à une température de 40 °C ; • - la dissolution du résidu sec dans 50 µl de tampon pour échantillon ; et • - l'analyse électrophorétique (par MEKC, chromatographie électrocinétique micellaire).
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)