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1. (WO2019050390) DERMAL FIBROBLAST CONDITIONED SERA BASED ON DEFINED KERATINOCYTE-SPECIFIC MEDIUM FOR SKIN REGENERATION AND REJUVENATION
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Pub. No.: WO/2019/050390 International Application No.: PCT/MY2018/050056
Publication Date: 14.03.2019 International Filing Date: 30.08.2018
IPC:
C12N 5/077 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
Applicants:
UNIVERSITI KEBANGSAAN MALAYSIA [MY/MY]; 43600 Ukm Bangi Selangor, MY
Inventors:
ROY CHOWDHURY, Shiplu; MY
BINTI MAAROF, Manira; MY
BINTI HAJI ABDUL GHANI, Nurul 'izzah; MY
BINTI HAJI IDRUS, Ruszymah; MY
Agent:
LOK, Choon Hong; MY
Priority Data:
PI 201770328707.09.2017MY
Title (EN) DERMAL FIBROBLAST CONDITIONED SERA BASED ON DEFINED KERATINOCYTE-SPECIFIC MEDIUM FOR SKIN REGENERATION AND REJUVENATION
(FR) SÉRUMS CONDITIONNÉS DE FIBROBLASTES DERMIQUES BASÉS SUR UN MILIEU DÉFINI SPÉCIFIQUE DE KÉRATINOCYTES POUR LA RÉGÉNÉRATION ET LE RAJEUNISSEMENT DE LA PEAU
Abstract:
(EN) This invention relates to a method of producing a medium conditioned using dermal fibroblasts in a defined keratinocyte-specific medium, and a skin rejuvenation composition derived thereof. More particularly, the method of preparing a conditioned cell culture medium, comprising the steps of: culturing dermal fibroblast cells in a culture medium comprising a serum-free keratinocyte-specific medium supplemented with stimulatory factors until the medium contains fibroblast secreted proteins of fibronectin, serum albumin, collagen alpha-1(VI) chain, collagen alpha-1(I) chain, decorin, serotransferrin, pentraxin- related protein PTX3 and metalloproteinase inhibitor 1.
(FR) La présente invention concerne un procédé de production d'un milieu conditionné à l'aide de fibroblastes dermiques dans un milieu défini spécifique aux kératinocytes, et une composition de rajeunissement de la peau dérivée de celui-ci. Plus particulièrement, le procédé de préparation d'un milieu de culture cellulaire conditionné comprend les étapes consistant à : la culture de cellules fibroblastiques dermiques dans un milieu de culture comprenant un milieu spécifique de kératinocytes exempt de sérum additionné de facteurs stimulateurs jusqu'à ce que le milieu contienne des protéines sécrétées par les fibroblastes de la fibronectine, de l'albumine sérique, de la chaine alpha-1 du collagène VI, chaine alpha-1 du collagène I, de la décorine, de la sérotransferrine, de la protéine apparentée à pentraxine PTX3 et de l’inhibiteur de métalloprotéinase 1.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)