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1. (WO2019050318) METHOD FOR INCREASING SECRETION OF RECOMBINANT PROTEIN
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Pub. No.: WO/2019/050318 International Application No.: PCT/KR2018/010466
Publication Date: 14.03.2019 International Filing Date: 07.09.2018
IPC:
C12P 21/00 (2006.01) ,C12N 9/24 (2006.01) ,C07K 14/415 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415
from plants
Applicants:
한국과학기술원 KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY [KR/KR]; 대전시 유성구 대학로 291 291, Daehak-ro Yuseong-gu Daejeon 34141, KR
Inventors:
안정훈 AHN, Jung Hoon; KR
변현종 BYUN, Hyun Jong; KR
박지연 PARK, Ji Yeon; KR
Agent:
유미특허법인 YOU ME PATENT AND LAW FIRM; 서울시 강남구 테헤란로 115 115 Teheran-ro Gangnam-gu Seoul 06134, KR
Priority Data:
10-2017-011481307.09.2017KR
10-2018-003157919.03.2018KR
Title (EN) METHOD FOR INCREASING SECRETION OF RECOMBINANT PROTEIN
(FR) PROCÉDÉ D'AUGMENTATION DE LA SÉCRÉTION DE PROTÉINE RECOMBINANTE
(KO) 재조합 단백질의 분비를 증가시키는 방법
Abstract:
(EN) The present invention relates to a method for effective extracellular secretion of a target protein, wherein the target protein is decreased in pI by adjusting the overall charge thereof and linked to LARD3 to prepare a fusion protein and a bacterial type 1 secretion system (T1SS) ABC transporter is employed. When used, the method allows the mass production of a protein simply and effectively without a separate purification process.
(FR) La présente invention concerne un procédé de sécrétion extracellulaire efficace d'une protéine cible, le pl de la protéine cible étant réduit par ajustement de sa charge globale et la protéine étant liée à LARD3 pour préparer une protéine de fusion et un transporteur ABC de système de sécrétion de type 1 (T1SS) bactérien étant employé. Le procédé permet, lorsqu'il est utilisé, la production de masse d'une protéine simplement et efficacement sans processus de purification séparé.
(KO) 본 발명은 목적 단백질의 전체적인 전하를 조절해 pI를 낮춘 목적단백질과 LARD3가 연결된 융합 단백질을 제조하여, 세균의 타입 1 분비 시스템 (Type 1 Secretion System, T1SS)의 ABC수송체를 이용해 목적 단백질을 효과적으로 세포 외 분비시키는 방법에 관한 것이다. 상기 방법을 사용해 별다른 정제과정을 거치지 않고서도 간단하고 효율적으로 단백질을 대량생산할 수 있다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)