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1. (WO2019050222) METHOD FOR SIMPLY AND EFFICIENTLY ISOLATING AND PURIFYING CHEMICALLY LABELED OLIGONUCLEOTIDES BY MEANS OF PH CONTROL
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Pub. No.: WO/2019/050222 International Application No.: PCT/KR2018/010153
Publication Date: 14.03.2019 International Filing Date: 31.08.2018
IPC:
C12Q 1/6806 (2018.01) ,C12N 15/10 (2006.01)
[IPC code unknown for C12Q 1/6806]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
서울대학교산학협력단 SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION [KR/KR]; 서울시 관악구 관악로 1 1, Gwanak-ro Gwanak-gu Seoul 08826, KR
Inventors:
박소영 BAK, So Young; KR
황지희 HWANG, Jihee; KR
김성근 KIM, Seong Keun; KR
김영규 KIM, Youngyu; KR
임순규 IM, Soonkyu; KR
Agent:
특허법인 공간 GHONG-GAN INTERNATIONAL PATENT LAW FIRM; 서울시 강남구 논현로 412(역삼동, 평화빌딩) 402호 #402, (Yeoksam-dong Pyunghwa B/D) 412, Nonhyeon-ro Gangnam-gu Seoul 06224, KR
Priority Data:
10-2017-011487508.09.2017KR
Title (EN) METHOD FOR SIMPLY AND EFFICIENTLY ISOLATING AND PURIFYING CHEMICALLY LABELED OLIGONUCLEOTIDES BY MEANS OF PH CONTROL
(FR) PROCÉDÉ PERMETTANT D'ISOLER ET DE PURIFIER DE FAÇON SIMPLE ET EFFICACE DES OLIGONUCLÉOTIDES MARQUÉS CHIMIQUEMENT AU MOYEN D'UNE RÉGULATION DE PH
(KO) pH 제어에 의한 화학적으로 표지된 올리고 핵산의 간편하고 효율적인 분리정제법
Abstract:
(EN) The present invention relates to a method for simply and efficiently isolating and purifying chemically labeled oligonucleotides by means of pH control and, more specifically, to a method and a kit for efficiently isolating and purifying labeled nucleotides by using a change in the distribution constant of a chemical marker according to the pH control of a reaction solution.
(FR) L'invention concerne un procédé permettant d'isoler et de purifier de façon simple et efficace des oligonucléotides marqués chimiquement, au moyen d'une régulation de pH et, plus spécifiquement, un procédé et un kit permettant d'isoler et de purifier efficacement des nucléotides marqués, par un changement de la constante de distribution d'un marqueur chimique en fonction de la régulation de pH d'une solution de réaction.
(KO) 본 발명은 pH 제어에 의한 화학적으로 표지된 올리고 핵산의 간편하고 효율적인 분리정제법에 관한 것으로, 보다 상세하게는 반응용액의 pH 조절에 따른 화학적 표지자의 분배상수 변화를 이용하여 표지화된 핵산을 효율적으로 분리 정제하는 방법 및 키트에 관한 것이다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)