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1. (WO2019048026) DENDRITIC CELL POTENCY ASSAY
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/048026 International Application No.: PCT/EP2017/072254
Publication Date: 14.03.2019 International Filing Date: 05.09.2017
IPC:
G01N 33/68 (2006.01) ,C12N 5/0784 (2010.01) ,A61K 35/15 (2015.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0784
Dendritic cells; Progenitors thereof
[IPC code unknown for A61K 35/15]
Applicants:
MEDIGENE IMMUNOTHERAPIES GMBH [DE/DE]; Lochhamer Straße 11 82152 Planegg-Martinsried, DE
Inventors:
SCHENDEL, Dolores; DE
ECKL, Judith; DE
GEIGER, Christiane; DE
RÖMER, Isabel; DE
Agent:
MAIWALD PATENTANWALTS- UND RECHTSANWALTSGESELLSCHAFT MBH; Annelie Wünsche Postfach 33 05 23 80065 München, DE
Priority Data:
Title (EN) DENDRITIC CELL POTENCY ASSAY
(FR) TEST D'ACTIVITÉ BIOLOGIQUE DE CELLULE DENDRITIQUE
Abstract:
(EN) The present invention relates to a method for determining the potency of DCs, comprising the steps: stimulating dendritic cells by incubation with soluble CD40L and TLR7/8 agonist, measuring the secretion of the marker proteins IL-10 and IL-12 from the stimulated dendritic cells. Thereby it can be determined whether the dendritic cell have a high capability to activate T-cells and Natural Killer (NK) cells. The invention also encompasses a method for stimulating dendritic cells comprising the step of stimulating the dendritic cells with soluble CD40L and TLR7/8 agonist.
(FR) La présente invention concerne un procédé de détermination de l'activité biologique de CD consistant : à stimuler des cellules dendritiques par incubation avec du CD40L soluble et un agoniste de TLR7/8, à mesurer la sécrétion des protéines marqueurs IL-10 et IL-12 à partir des cellules dendritiques stimulées. Ainsi, il est possible de déterminer si la cellule dendritique présente une capacité élevée à activer les lymphocytes T et les cellules tueuses naturelles (NK). L'invention concerne également un procédé de stimulation de cellules dendritiques consistant à stimuler les cellules dendritiques avec du CD40L soluble et un agoniste de TLR7/8.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)