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1. (WO2019046703) METHODS FOR IMPROVING GENOME EDITING IN FUNGI
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Pub. No.: WO/2019/046703 International Application No.: PCT/US2018/049066
Publication Date: 07.03.2019 International Filing Date: 31.08.2018
IPC:
C12N 15/01 (2006.01) ,C12N 15/63 (2006.01) ,C12N 15/80 (2006.01) ,C12N 15/90 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01
Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
Applicants:
SHASKY, Jeffrey [US/US]; US (BW)
NOVOZYMES A/S [DK/DK]; Krogshoejvej 36 DK-2880 Bagsvaerd, DK
Inventors:
SHASKY, Jeffrey; US
MAIYURAN, Suchindra; US
LEHMBECK, Jan; DK
Agent:
STARNES, Robert; US
FECHTER, Eric; US
LAMBIRIS, Elias; US
REYNOLDS, Kelly; US
SLADEK, Todd; US
FAZZOLARE, David; US
Priority Data:
62/553,37401.09.2017US
Title (EN) METHODS FOR IMPROVING GENOME EDITING IN FUNGI
(FR) PROCÉDÉS D'AMÉLIORATION DE L'ÉDITION DU GÉNOME DANS DES CHAMPIGNONS
Abstract:
(EN) The present disclosure relates to methods for modifying the genome of a fungal cell, comprising: (A) introducing into the fungal cell: (i) a first nucleic acid construct comprising a polynucleotide encoding an RNA-guided DNA endonuclease; (ii) a second nucleic acid construct comprising (a) a U6 promoter sequence operably linked to (1) a transfer RNA encoding sequence, (2) a single guide RNA encoding sequence, and (b) a U6 terminator sequence; and optionally (iii) a third nucleic acid construct comprising a donor DNA for modifying the genome; and (B) selecting a transformant of the fungal cell, wherein the genome is modified.
(FR) La présente invention concerne des procédés destinés à modifier le génome d'une cellule fongique, comprenant les étapes consistant : (A) à introduire dans la cellule fongique : (i) une première construction d'acide nucléique comprenant un polynucléotide codant pour une endonucléase d'ADN guidée par ARN ; (ii) une deuxième construction d'acide nucléique comprenant (a) une séquence de promoteur U6 liée fonctionnellement à (1) une séquence de codage d'ARN de transfert, (2) une séquence de codage d'ARN de guidage unique, et (b) une séquence de terminaison U6 ; et éventuellement (iii) une troisième construction d'acide nucléique comprenant un ADN donneur destiné à modifier le génome ; et (B) à sélectionner un transformant de la cellule fongique, le génome étant modifié.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)