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1. (WO2019046446) COMPOSITIONS AND METHODS USING METHANOTROPHIC S-LAYER PROTEINS FOR EXPRESSION OF HETEROLOGOUS PROTEINS
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Pub. No.: WO/2019/046446 International Application No.: PCT/US2018/048576
Publication Date: 07.03.2019 International Filing Date: 29.08.2018
IPC:
C12N 1/20 (2006.01) ,C12N 15/62 (2006.01) ,C12N 15/74 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
62
DNA sequences coding for fusion proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
Applicants:
SAN DIEGO STATE UNIVERSITY (SDSU) FOUNDATION, DBA SAN DIEGO STATE UNIVERSITY RESEARCH FOUNDATION [US/US]; 5500 Campanile Drive Mail Code 8220 San Diego, California 92182, US
Inventors:
KALYUZHNAYA, Marina; US
DEMIDENKO, Oleksandr; US
COLLINS, David; US
Agent:
EINHORN, Gregory P.; US
Priority Data:
62/551,50229.08.2017US
62/551,59029.08.2017US
Title (EN) COMPOSITIONS AND METHODS USING METHANOTROPHIC S-LAYER PROTEINS FOR EXPRESSION OF HETEROLOGOUS PROTEINS
(FR) COMPOSITIONS ET PROCÉDÉS UTILISANT DES PROTÉINES DE COUCHE S MÉTHANOTROPHE POUR L'EXPRESSION DE PROTÉINES HÉTÉROLOGUES
Abstract:
(EN) In alternative embodiments, provided are compositions and methods for making a chimeric polypeptide comprising an S-layer polypeptide and a heterologous polypeptide or peptide. In alternative embodiments, the compositions and methods comprise recombinantly engineering a methylotrophic or methanotrophic bacteria to recombinantly express a chimeric polypeptide comprising an S-layer polypeptide and a heterologous polypeptide or peptide. Also provided are compositions and methods for displaying or immobilizing proteins on a methanotrophic S-layer. In alternative embodiments, provided are compositions and methods comprising recombinant methylotrophic or methanotrophic bacteria comprising assembled or self-assembled recombinant or isolated chimeric S-layer polypeptides. In alternative embodiments, provided are compositions and methods using recombinant methylotrophic or methanotrophic bacteria, optionally a Methylomicrobium alcaliphilum, optionally a M. alcaliphilum sp. 20Z, for ectoine ((4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), for the production or synthesis of a protein, e.g., an ectoine, or an enzyme, e.g., a lipase.
(FR) L'invention concerne, selon divers modes de réalisation, des compositions et des procédés pour la fabrication d'un polypeptide chimère comprenant un polypeptide de couche S et un polypeptide ou un peptide hétérologue. Dans d'autres modes de réalisation, les compositions et les procédés comprennent la manipulation par recombinaison d'une bactérie méthylotrophe ou méthanotrophe pour exprimer par recombinaison un polypeptide chimère comprenant un polypeptide de couche S et un polypeptide ou un peptide hétérologue. L'invention concerne également des compositions et des procédés pour l'affichage ou l'immobilisation de protéines sur une couche S méthanotrophe. Dans d'autres modes de réalisation, l'invention concerne des compositions et des procédés comprenant des bactéries méthylotrophes ou méthanotrophes recombinées comprenant des polypeptides chimères de couche S assemblés ou auto-assemblés, recombinés ou isolés. Dans d'autres modes de réalisation, l'invention concerne des compositions et des procédés utilisant des bactéries méthylotrophes ou méthanotrophes recombinées, éventuellement une bactérie Methylomicrobium alcaliphilum, éventuellement une bactérie M. alcaliphilum sp. 20Z, pour l'ectoïne (acide (4S)-2-méthyl-1,4,5,6-tétrahydropyrimidine-4 carboxylique), pour la production ou la synthèse d'une protéine, par exemple une ectoïne, ou d'une enzyme, par exemple une lipase.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)