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1. (WO2019046288) COMPOSITIONS AND MULTIPLEX ASSAYS FOR CHARACTERIZING ACTIVE PROTEASES AND THEIR INHIBITORS
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/046288 International Application No.: PCT/US2018/048321
Publication Date: 07.03.2019 International Filing Date: 28.08.2018
IPC:
C12Q 1/37 (2006.01) ,G01N 33/543 (2006.01) ,G01N 33/573 (2006.01) ,G01N 33/58 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
37
involving peptidase or proteinase
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
573
for enzymes or isoenzymes
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58
involving labelled substances
Applicants:
MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US/US]; 77 Massachusetts Avenue Cambridge, Massachusetts 02139, US
Inventors:
AHRENS, Caroline Chopko; US
GRIFFITH, Linda G.; US
TANNENBAUM, Steven Robert; US
COOK, Christi Dionne; US
KODIHALLI, Ravindra; US
LAUFFENBURGER, Douglas A.; US
CHISWICK, Evan L.; US
MILLER, Miles; US
Agent:
PABST, Patrea L.; US
MONHEIT, Rivka D.; US
SHYNTUM, Yvonne Y.; US
HODGES, Robert A.; US
RAYMOND, Adam S.; US
YONGYE, Austin B.; US
SARGSYAN, S. Anna; US
ZUNIGA, Carlos A.; US
Priority Data:
62/552,77331.08.2017US
62/585,91814.11.2017US
Title (EN) COMPOSITIONS AND MULTIPLEX ASSAYS FOR CHARACTERIZING ACTIVE PROTEASES AND THEIR INHIBITORS
(FR) COMPOSITIONS ET DOSAGES MULTIPLEXES POUR CARACTÉRISER DES PROTÉASES ACTIVES ET LEURS INHIBITEURS
Abstract:
(EN) Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 µl) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies. It also allows for high throughput screening to identify inhibitors of proteases in complex, donor-derived samples.
(FR) Les protéases régulent une large gamme de fonctions cellulaires normales dans lesquelles une activité dérégulée est observée dans diverses maladies. Les compositions et les procédés utilisent des immunodosages à base de billes, multiplexés pour activité de protéase en vue de profiler l'activité de la protéase. Cette technologie de plateforme intègre des mesures d'activité de protéase avec des techniques de quantification de protéines totales. Elle représente une amélioration significative par rapport aux techniques de détection existantes en permettant des mesures de protéases actives sensibles multiplexées dans des échantillons biologiques complexes. Des exemples de détections multiplexées sont réalisés dans un dosage unique à l'aide d'une petite quantité d'échantillon (par exemple 5 µl) pour MMP-1, MMP-2, MMP-3, MMP-7, MMP-9 et MMP-12 recombinants actifs et ces mêmes MMP dans une phase surnageante de culture cellulaire, un effluent de fluide menstruel et des aspirations péritonéales. Cette plateforme multiplexée permet d'obtenir un niveau élevé de sensibilités, égal ou supérieur à celui des principales stratégies de détection monoplexées existantes. Elle permet également un criblage à haut début pour identifier des inhibiteurs de protéases dans des échantillons complexes, dérivés du donneur.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)