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1. (WO2019046195) METHODS AND COMPOSITIONS FOR THE PRESERVATION OF TISSUE
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Pub. No.: WO/2019/046195 International Application No.: PCT/US2018/048156
Publication Date: 07.03.2019 International Filing Date: 27.08.2018
IPC:
A01N 1/00 (2006.01) ,A01N 1/02 (2006.01) ,A61K 35/12 (2015.01) ,A61K 35/14 (2015.01) ,A61K 35/19 (2015.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
02
Preservation of living parts
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
14
Blood
[IPC code unknown for A61K 35/19]
Applicants:
FORM 62 LLC [US/US]; 2260 Clove Drive San Jose, California 95128, US
Inventors:
MCINTYRE, Robert; US
MCCANNA, Michael; US
Agent:
WEINER, Ethan; US
Priority Data:
62/550,94528.08.2017US
Title (EN) METHODS AND COMPOSITIONS FOR THE PRESERVATION OF TISSUE
(FR) PROCÉDÉS ET COMPOSITIONS POUR LA CONSERVATION DE TISSU
Abstract:
(EN) Described herein are methods of preserving a tissue of a subject, such as a brain or a portion thereof. The methods include washing the tissue by perfusing the tissue with a wash fluid comprising an aqueous solution; fixing the tissue by perfusing the tissue with a fixation fluid comprising an aldehyde; and cryoprotecting the tissue by perfusing the tissue with a cryoprotection fluid comprising a vitrification agent. The wash fluid, the fixation fluid, and/or the cryoprotection fluid can include a dye or a contrast agent to monitor perfusion of the fluid through the tissue. In certain embodiments, the cryoprotection fluid has a vitrification temperature of about -80 °C or higher. The wash fluid can further include one or more of an ion channel blocker, a calcium chelator, a thrombolytic agent, an anti-platelet, a respiratory poison, or a synaptic poison. Also described herein are methods of analyzing the preserved tissue.
(FR) La présente invention concerne des procédés de conservation d’un tissu d’un sujet, tel qu’un cerveau ou une partie de celui-ci. Les procédés comprennent le lavage du tissu par perfusion du tissu avec un fluide de lavage comprenant une solution aqueuse ; la fixation du tissu par perfusion du tissu avec un fluide de fixation comprenant un aldéhyde ; et la cryoprotection du tissu par perfusion du tissu avec un fluide de cryoprotection comprenant un agent de vitrification. Le fluide de lavage, le fluide de fixation et/ou le fluide de cryoprotection peuvent comprendre un colorant ou un agent de contraste pour surveiller la perfusion du fluide à travers le tissu. Dans certains modes de réalisation, le fluide de cryoprotection a une température de vitrification d’environ -80 °C ou plus. Le fluide de lavage peut comprendre un en outre ou plusieurs agents parmi un bloqueur de canal ionique, un chélateur de calcium, un agent thrombolytique, un antiplaquettaire, un poison respiratoire ou un poison synaptique. L’invention concerne en outre des procédés d’analyse du tissu conservé.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)