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1. (WO2019045649) METHODS FOR ENRICHING MESENCHYMAL STEM CELLS
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Pub. No.: WO/2019/045649 International Application No.: PCT/SG2018/050438
Publication Date: 07.03.2019 International Filing Date: 29.08.2018
IPC:
C12N 5/0775 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
Applicants:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way #20-10 Connexis North Tower Singapore 138632, SG
Inventors:
LIAU, Brian; SG
RAGHOTHAMAN, Deepak; SG
OH, Steve; SG
Agent:
SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531 Robinson Road Post Office Singapore 903031, SG
Priority Data:
10201707052R29.08.2017SG
Title (EN) METHODS FOR ENRICHING MESENCHYMAL STEM CELLS
(FR) MÉTHODES POUR ENRICHIR DES CELLULES SOUCHES MÉSENCHYMATEUSES
Abstract:
(EN) Methods of enriching non-senescent mesenchymal stem cells (MSCs) and methods of performing quality control for non-senescent MSCs are disclosed. The methods comprise contacting a sample comprising MSCs with a probe (specifically peanut agglutinin, PNA) that binds to a glycan (specifically glycan T-antigen) expressed on the surface of MSCs, with the separation or determination of non-senescent MSCs from senescent MSCs based on a difference in expression of the glycan at the cell surface, i.e. the non-senescent MSCs express more of the glycan than senescent MSCs. A high amount of non-senescent MSCs is indicative of a sample of acceptable quality.
(FR) L'invention concerne des méthodes pour enrichir des cellules souches mésenchymateuses (MSC) non sénescentes, ainsi que des méthodes permettant de réaliser un contrôle qualité portant sur les MSC non sénescentes. Les méthodes comprennent la mise en contact d'un échantillon comprenant des MSC avec une sonde (spécifiquement l'agglutinine d'arachide, PNA) qui se lie à un glycane (spécifiquement le glycane antigène T) exprimé en surface des MSC, la séparation ou la détermination des MSC non sénescentes des MSC sénescentes se fondant sur la différence d'expression du glycane au niveau de la surface cellulaire, les MSC non sénescentes exprimant une plus grande quantité du glycane que les MSC sénescentes. Une quantité élevée de MSC non sénescentes indique un échantillon de qualité acceptable.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)