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1. (WO2019045079) METHOD FOR MEASURING CHOLESTEROL EFFLUX CAPACITY
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Pub. No.: WO/2019/045079 International Application No.: PCT/JP2018/032500
Publication Date: 07.03.2019 International Filing Date: 31.08.2018
IPC:
C12Q 1/60 (2006.01) ,C12Q 1/02 (2006.01) ,G01N 27/62 (2006.01) ,G01N 33/92 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
60
involving cholesterol
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
62
by investigating the ionisation of gases; by investigating electric discharges, e.g. emission of cathode
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
92
involving lipids, e.g. cholesterol
Applicants:
積水メディカル株式会社 SEKISUI MEDICAL CO., LTD. [JP/JP]; 東京都中央区日本橋二丁目1番3号 1-3, Nihonbashi 2-chome, Chuo-ku, Tokyo 1030027, JP
Inventors:
清水 知 SHIMIZU, Tomo; JP
臼井 智之 USUI, Tomoyuki; JP
宮崎 修 MIYAZAKI, Osamu; JP
吉田 博 YOSHIDA, Hiroshi; JP
Agent:
特許業務法人アルガ特許事務所 THE PATENT CORPORATE BODY ARUGA PATENT OFFICE; 東京都中央区日本橋人形町1丁目3番8号 沢の鶴人形町ビル Sawanotsuru Ningyocho Bldg., 1-3-8, Nihonbashi Ningyocho, Chuo-ku, Tokyo 1030013, JP
Priority Data:
2017-16808331.08.2017JP
2017-17256307.09.2017JP
2017-19552205.10.2017JP
Title (EN) METHOD FOR MEASURING CHOLESTEROL EFFLUX CAPACITY
(FR) MÉTHODE DE MESURE DE LA CAPACITÉ D'EFFLUX DE CHOLESTÉROL
(JA) コレステロール引き抜き能の測定方法
Abstract:
(EN) Provided is a method for measuring cholesterol efflux capacity that can satisfy clinical requirements. The method for measuring the cholesterol efflux capacity of a test substance includes: (1) a step for adding a stable isotope-labeled cholesterol to a cell-containing culture medium and culturing to bring about the uptake of the stable isotope-labeled cholesterol by the cells; (2) a step for culturing, in the presence of a test substance, the cells that have uptaken the cholesterol; and (3) a step for measuring the amount of stable isotope-labeled cholesterol effluxed from the cells.
(FR) L'invention concerne une méthode de mesure de la capacité d'efflux de cholestérol qui peut satisfaire des exigences cliniques. La méthode de mesure de la capacité d'efflux de cholestérol d'une substance d'essai comprend : (1) une étape d'ajout d'un cholestérol marqué par un isotope stable à un milieu de culture contenant des cellules et la culture pour provoquer l'absorption du cholestérol marqué par un isotope stable par les cellules ; (2) une étape de culture, en présence d'une substance d'essai, des cellules qui ont absorbé le cholestérol ; et (3) une étape de mesure de la quantité de cholestérol marqué par un isotope stable excrété par les cellules.
(JA) 臨床上の要求を満足することができるコレステロール引き抜き能の測定方法。(1)細胞を含む培養液に安定同位体標識されたコレステロールを添加して培養し、該細胞に該安定同位体標識されたコレステロールを取り込ませる工程、(2)該コレステロールを取り込ませた細胞を、被験物質存在下で培養する工程、(3)該細胞から引き抜かれた安定同位体標識されたコレステロールの量を測定する工程、を含む被験物質のコレステロール引き抜き能の測定方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)