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1. (WO2019045016) HIGH-SENSITIVITY, QUANTITATIVE GENE EXAMINATION METHOD, PRIMER SET, AND EXAMINATION KIT
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Pub. No.: WO/2019/045016 International Application No.: PCT/JP2018/032260
Publication Date: 07.03.2019 International Filing Date: 30.08.2018
IPC:
C12Q 1/686 (2018.01) ,C12Q 1/6876 (2018.01) ,C12N 15/09 (2006.01)
[IPC code unknown for C12Q 1/686][IPC code unknown for C12Q 1/6876]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
公益財団法人がん研究会 JAPANESE FOUNDATION FOR CANCER RESEARCH [JP/JP]; 東京都江東区有明三丁目8番31号 8-31, Ariake 3-chome, Koto-ku, Tokyo 1358550, JP
Inventors:
野村 幸男 NOMURA Sachio; JP
新井 正美 ARAI Masami; JP
Agent:
白形 由美子 SHIRAKATA Yumiko; JP
Priority Data:
2017-16807831.08.2017JP
Title (EN) HIGH-SENSITIVITY, QUANTITATIVE GENE EXAMINATION METHOD, PRIMER SET, AND EXAMINATION KIT
(FR) PROCÉDÉ D'EXAMEN QUANTITATIF À HAUTE SENSIBILITÉ DE GÈNE, ENSEMBLE D'AMORCES ET KIT D'EXAMEN
(JA) 高感度かつ定量的な遺伝子検査方法、プライマーセット、及び検査キット
Abstract:
(EN) According to the present invention, the DNA in a sample can be highly sensitively and quantitatively detected by: designing primers that are for the detection of methylated DNA and unmethylated DNA or wild-type and mutated genes such that PCR products have different Tm values; and analyzing melting curves.
(FR) Selon la présente invention, l'ADN dans un échantillon peut être détecté de manière hautement sensible et quantitative par : la conception d'amorces qui sont conçues pour la détection d'ADN méthylé et d'ADN non méthylé ou de gènes de type sauvage et mutés, de telle sorte que les produits de PCR présentent des valeurs Tm différentes ; et l'analyse de courbes de fusion.
(JA) メチル化DNAと非メチル化DNA、又は野生型と変異型遺伝子を検出するプライマーをPCR産物のTm値が異なるように設計し、融解曲線を分析することにより、高感度かつ定量的にサンプル中のDNAを検出することができる。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)