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1. (WO2019041635) NUCLEIC ACID CONSTRUCT FOR ENDOGENOUS EXPRESSION RNA POLYMERASE IN CELLS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/041635 International Application No.: PCT/CN2017/115967
Publication Date: 07.03.2019 International Filing Date: 13.12.2017
IPC:
C12N 15/81 (2006.01) ,C12N 1/19 (2006.01) ,C12R 1/645 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
81
for yeasts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
645
Fungi
Applicants:
康码(上海)生物科技有限公司 KANGMA (SHANGHAI) BIOTECH COR. LTD. [CN/CN]; 中国上海市 浦东新区中国(上海)自由贸易试验区蔡伦路781号1311室 Room 1311, No. 781 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area Shanghai 201203, CN
Inventors:
郭敏 GUO, Min; CN
代田纯 DAI, Tianchun; CN
薛银鸽 XUE, Yin'ge; CN
李海洋 LI, Haiyang; CN
王海鹏 WANG, Haipeng; CN
柴智 CHAI, Zhi; CN
刘帅龙 LIU, Shuailong; CN
于雪 YU, Xue; CN
Agent:
上海一平知识产权代理有限公司 XU & PARTNERS, LLC.; 中国上海市 普陀区真北路958号天地科技广场1号楼106室 Room No. 106, Building No. 1, Universal High-Tech Plaza, 958 Zhen Bei Road, Putuo District Shanghai 200333, CN
Priority Data:
201710768550.131.08.2017CN
Title (EN) NUCLEIC ACID CONSTRUCT FOR ENDOGENOUS EXPRESSION RNA POLYMERASE IN CELLS
(FR) CONSTRUCTION D'ACIDE NUCLÉIQUE POUR ARN POLYMÉRASE D'EXPRESSION ENDOGÈNE DANS DES CELLULES
(ZH) 一种细胞中内源性表达RNA聚合酶的核酸构建物
Abstract:
(EN) Provided is a nucleic acid construct for endogenous expression RNA polymerase in cells, the nucleic acid construct consisting of an encoding sequence of a promoter (such as RNR2, ADH1, GAPDH, TEF1, PGK1, SED1 or a combination thereof) having specific promotion strength and RNA polymerase (such as T7RNAP, T3RNAP, T4RNAP, T5RNAP or a combination thereof), and being used for synthesizing foreign proteins in an in vitro yeast protein synthesis system.
(FR) L'invention concerne une construction d'acide nucléique pour une ARN polymérase d'expression endogène dans des cellules, la construction d'acide nucléique étant constituée d'une séquence codante d'un promoteur (tel que RNR2, ADH1, GAPDH, TEF1, PGK1, SED1 ou une combinaison de ceux-ci) ayant une résistance de promotion spécifique et une ARN polymérase (telle que T7RNAP, T3RNAP, T4RNAP, T5RNAP ou une combinaison de ceux-ci), et étant utilisée pour synthétiser des protéines étrangères dans un système de synthèse de protéine de levure in vitro.
(ZH) 提供了一种细胞中内源性表达RNA聚合酶的核酸构建物,该核酸构建物由具有特定启动强度的启动子(如RNR2、ADH1、GAPDH、TEF1、PGK1、SED1、或其组合)和RNA聚合酶 (如T7RNAP、T3RNAP、T4RNAP、T5RNAP、或其组合)的编码序列构成,用于在酵母体外蛋白质合成体系中合成外源蛋白。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)