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1. (WO2019041378) FETAL BOVINE SERUM SUBSTITUTE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
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Pub. No.: WO/2019/041378 International Application No.: PCT/CN2017/101552
Publication Date: 07.03.2019 International Filing Date: 13.09.2017
IPC:
C12N 5/00 (2006.01) ,C12N 5/02 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02
Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
Applicants:
广州蕊特生物科技有限公司 GUANGZHOU RUITE BIOTECHNOLOGY CO., LTD. [CN/CN]; 中国广东省广州市 科学城揽月路3号广州国际企业孵化器F栋105号房 Room 105, Building F, GIBI, No. 3, Lanyue Road, Science City Guangzhou, Guangdong 510663, CN
Inventors:
李长路 LI, Changlu; CN
Agent:
广州嘉权专利商标事务所有限公司 JIAQUAN IP LAW FIRM; 中国广东省广州市 天河区黄埔大道西100号富力盈泰广场A栋910张萍 ZHANG, Ping No.910, Building A, Winner Plaza, No.100, West Huangpu Avenue, Tianhe District Guangzhou, Guangdong 510627, CN
Priority Data:
201710771221.231.08.2017CN
Title (EN) FETAL BOVINE SERUM SUBSTITUTE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
(FR) SUBSTITUT DE SÉRUM BOVIN FŒTAL, SON PROCÉDÉ DE PRÉPARATION, ET SON APPLICATION
(ZH) 一种胎牛血清替代物及其制备方法和应用
Abstract:
(EN) A fetal bovine serum substitute, a preparation method therefor, and an application thereof in a culture medium. The fetal bovine serum substitute is prepared by adding nutrient elements to a protein-depleted bovine serum. The nutrients consist of a specific ratio of CaCl2•2H2O, CuSO4•5H2O, 1% ferrous citrate, hydrocortisone, FeSO4•7H2O, KCl, MgCl2•6H2O, MgSO4•7H2O, NaCl, NaH2PO4•2H2O, Na2HPO4•12H2O, glucose, sodium pyruvate, a GABA stock solution, a LiCl stock solution, a piperidic acid concentrate, a Stock A stock solution, and a Na2SeO3 stock solution.
(FR) L'invention concerne un substitut de sérum bovin fœtal, son procédé de préparation, et son application dans un milieu de culture. Le substitut de sérum bovin fœtal est préparé par ajout de nutriments à un sérum bovin appauvri en protéines. Les nutriments consistent en un rapport spécifique de CaCl2•2H2O, de CuSO4•5H2O, de citrate ferreux 1 %, d'hydrocortisone, de FeSO4•7H2O, de KCl, de MgCl2•6H2O, de MgSO4•7H2O, de NaCl, de NaH2PO4•2H2O, de Na2HPO4•12H2O, de glucose, de pyruvate de sodium, d'une solution mère GABA, d'une solution mère LiCl, d'un concentré d'acide pipéridique, d'une solution mère Stock A, et d'une solution mère de Na2SeO3.
(ZH) 一种胎牛血清替代物、制备方法及其在培养基中的应用。所述胎牛血清替代物通过在去除蛋白的牛血清中加入营养元素而制得。其中营养素由CaCl2•2H2O、CuSO4•5H2O、1%柠檬酸亚铁、氢化可的松、FeSO4•7H2O、KCl、MgCl2•6H2O、MgSO4•7H2O、NaCl、NaH2PO4•2H2O、Na2HPO4•12H2O、葡萄糖、丙酮酸钠、GABA储存液、LiCl储存液、哌啶酸浓缩液、Stock A储存液、Na2SeO3储存液按一定配比组成。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)