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1. (WO2019041066) CONSTRUCTION OF FH3 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF HIGH-EXPRESSION CELL STRAIN BY USING SAME
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Pub. No.: WO/2019/041066 International Application No.: PCT/CN2017/099188
Publication Date: 07.03.2019 International Filing Date: 26.08.2017
IPC:
C12N 15/113 (2010.01) ,C12N 15/64 (2006.01) ,C12N 15/12 (2006.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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DNA or RNA fragments; Modified forms thereof
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Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室毛吉炎 MAO, Jiyan Room 509, Building 1, Zhuguang Innovation Park No.134 Zhuguang Road, Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors:
毛吉炎 MAO, Jiyan; CN
Priority Data:
Title (EN) CONSTRUCTION OF FH3 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF HIGH-EXPRESSION CELL STRAIN BY USING SAME
(FR) CONSTRUCTION D'UN VECTEUR D'EXPRESSION EUCARYOTE FH3 ET PRÉPARATION D'UNE SOUCHE CELLULAIRE À HAUTE EXPRESSION À L'AIDE DE CELUI-CI
(ZH) 一种 FH3 真核表达载体的构建及其高表达细胞株的制备
Abstract:
(EN) Provided are construction of an eukaryotic expression vector and preparation of a stable-expression cell strain by using same. The method comprises: cloning an FH3 full-length gene to a pEGFP-N1 eukaryotic expression vector with a fluorescent protein tag; transfecting HAP-s cells by using the constructed eukaryotic expression vector; and establishing an FH3 stable-overexpression HAP-s cell line.
(FR) L'invention concerne la construction d'un vecteur d'expression eucaryote et la préparation d'une souche cellulaire à expression stable à l'aide de celui-ci. Le procédé comprend les étapes consistant à : cloner un gène FH3 de longueur complète sur un vecteur d'expression eucaryote pEGFP-N1 présentant une étiquette de protéine fluorescente; transfecter des cellules HAP-s à l'aide du vecteur d'expression eucaryote construit; et établir une lignée cellulaire HAP-s à surexpression stable de FH3.
(ZH) 提供了一种构建真核表达载体以及利用该载体制备稳定表达细胞株的方法,该方法为将FH3全长基因克隆至带有荧光蛋白标签的pEGFP-N1真核表达载体中,并用构建的真核表达载体转染HAP-s细胞,建立FH3稳定过表达的HAP-s细胞系。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)