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1. WO2019040583 - USE OF PHOSPHORYLATED TBETA4 AND OTHER FACTORS TO GENERATE HUMAN INDUCED PLURIPOTENT STEM CELLS

Publication Number WO/2019/040583
Publication Date 28.02.2019
International Application No. PCT/US2018/047458
International Filing Date 22.08.2018
IPC
C12N 5/074 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
074Adult stem cells
C07K 14/47 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
CPC
C07K 14/4702
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4702Regulators; Modulating activity
C07K 14/57581
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
575Hormones
57581Thymosin; Related peptides
C07K 2319/09
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
09containing a nuclear localisation signal
C07K 2319/10
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
10containing a tag for extracellular membrane crossing, e.g. TAT or VP22
C07K 5/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
C07K 7/08
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
7Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04Linear peptides containing only normal peptide links
08having 12 to 20 amino acids
Applicants
  • SO YOUNG LIFE SCIENCES CORPORATION [US]/[US]
  • SETH, Avinash [US]/[US]
Inventors
  • SETH, Avinash
Agents
  • FISH, Robert D.
  • KIM, Brian
  • PRIETO, Tomas
  • ANDELIN, Joseph
  • BRILLHART, Kurt
  • CASTANEDA, Steven
Priority Data
16/044,14224.07.2018US
62/550,33725.08.2017US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) USE OF PHOSPHORYLATED TBETA4 AND OTHER FACTORS TO GENERATE HUMAN INDUCED PLURIPOTENT STEM CELLS
(FR) UTILISATION DE TBÊTA4 PHOSPHORYLÉ ET AUTRES FACTEURS POUR GÉNÉRER DES CELLULES SOUCHES PLURIPOTENTES INDUITES HUMAINES
Abstract
(EN) The present invention provides compositions and methods for inducing pluripotency in non-embryonic and/or somatic cells using exogenous Tβ4, exogenous Sox2, and exogenous Oct4. Induced pluripotent stem cells can be can be simian or murine. The exogenous Tβ4 can be phosphorylated. At least one of the exogenous Tβ4, the exogenous Sox2, and the exogenous Oct4 can be coupled with a cell membrane penetrating moiety and/or a nuclear targeting moiety, allowing them to reach to the nucleus. In addition, the induced pluripotent stem cell maintains pluripotency over at least 100 passages.
(FR) La présente invention concerne des compositions et des procédés pour induire la pluripotence dans des cellules non embryonnaires et/ou somatiques à l'aide de Tβ4 exogène, Sox2 exogène et Oct4 exogène. Les cellules souches pluripotentes induites peuvent être simiennes ou murines. Le Tβ4 exogène peut être phosphorylé. Au moins un parmi les Tβ4 exogène, Sox2 exogène et Oct4 exogène peut être lié à un fragment de pénétration de membrane cellulaire et/ou à un fragment de ciblage nucléaire, leur permettant d'atteindre le noyau. De plus, la cellule souche pluripotente induite conserve sa pluripotence sur au moins 100 passages.
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