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1. (WO2019035335) NUCLEIC ACID COLLECTION METHOD, NUCLEIC-ACID-BINDING CARRIER, AND NUCLEIC ACID COLLECTION KIT
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/035335 International Application No.: PCT/JP2018/028130
Publication Date: 21.02.2019 International Filing Date: 26.07.2018
IPC:
C12N 15/10 (2006.01) ,C12M 1/00 (2006.01) ,C12M 1/26 (2006.01) ,B01D 15/00 (2006.01) ,B01J 20/10 (2006.01) ,B01J 20/28 (2006.01) ,B01J 20/34 (2006.01) ,C01B 33/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
26
Inoculator or sampler
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
02
comprising inorganic material
10
comprising silica or silicate
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
28
characterised by their form or physical properties
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
30
Processes for preparing, regenerating or reactivating
34
Regenerating or reactivating
C CHEMISTRY; METALLURGY
01
INORGANIC CHEMISTRY
B
NON-METALLIC ELEMENTS; COMPOUNDS THEREOF
33
Silicon; Compounds thereof
113
Silicon oxides; Hydrates thereof
12
Silica; Hydrates thereof, e.g. lepidoic silicic acid
Applicants:
AGC株式会社 AGC INC. [JP/JP]; 東京都千代田区丸の内一丁目5番1号 5-1, Marunouchi 1-chome, Chiyoda-ku, Tokyo 1008405, JP
Inventors:
酒井 智弘 SAKAI Tomohiro; JP
原 太志 HARA Futoshi; JP
小谷 哲也 KOTANI Tetsuya; JP
山▲崎▼ 信介 YAMASAKI Shinsuke; JP
Agent:
泉名 謙治 SENMYO Kenji; JP
小川 利春 OGAWA Toshiharu; JP
金 鎭文 KIM Jin-Moon; JP
比企野 健 HIKINO Ken; JP
横井 大一郎 YOKOI Daiichiro; JP
Priority Data:
2017-15796818.08.2017JP
2017-15797818.08.2017JP
Title (EN) NUCLEIC ACID COLLECTION METHOD, NUCLEIC-ACID-BINDING CARRIER, AND NUCLEIC ACID COLLECTION KIT
(FR) PROCÉDÉ DE COLLECTE D'ACIDES NUCLÉIQUES, SUPPORT POUR LIAISON D'ACIDES NUCLÉIQUES, ET KIT DE COLLECTE D'ACIDES NUCLÉIQUES
(JA) 核酸の回収方法、核酸結合用担体および核酸回収キット
Abstract:
(EN) Provided are: a method for collecting nucleic acid at a high rate of collection by means of a simple operation, even when the nucleic acid is long; a nucleic-acid-binding carrier that is suitable for use in the collection method; and a nucleic acid collection kit. A nucleic acid collection method that involves: forming composites by making nucleic acid bind to porous silica particles that have a D50 of 6–50 μm in a volume-based particle size distribution, a pore volume of 0.3–5 cm3/g as found by the BJH method, and an average pore diameter of 60–500 Å as found by the BJH method or porous silica particles that have a D50 of 6–50 μm in a volume-based particle size distribution, a pore volume of 0.6–5 cm3/g as found by mercury porosimetry, and an average pore diameter of 200–5,000 Å as found by mercury porosimetry; liquating the nucleic acid from the composites; and collecting the nucleic acid.
(FR) L'invention fournit un procédé destiné à collecter des acides nucléiques selon un rendement élevé par une manipulation simple, y compris dans le cas d'acides nucléiques longs, et un support pour liaison d'acides nucléiques ainsi qu'un kit de collecte d'acides nucléiques mettant en œuvre ledit procédé de collecte de manière adéquate. Plus précisément, l'invention concerne un procédé de collecte d'acides nucléiques selon lequel un complexe est formé par liaison à un acide nucléique soit de particules de silice poreuses de répartition granulométrique (D50) sur la base du volume comprise entre 6 et 50μm, de volume de pore obtenu selon un procédé BJH compris entre 0,3 et 5cm/g, et de diamètre de pore moyen obtenu selon le procédé BJH compris entre 60 et 500Å, soit de particules de silice poreuses de répartition granulométrique (D50) sur la base du volume comprise entre 6 et 50μm, de volume de pore obtenu par porosimétrie au mercure compris entre 0,6 et 5cm/g, et de diamètre de pore moyen obtenu par porosimétrie au mercure compris entre 200 et 5000Å. Enfin, selon le procédé de l'invention, ledit acide nucléique est soumis à une élution vis-à-vis dudit complexe et est collecté.
(JA) 核酸が長い場合でも、簡便な操作により高い回収率で核酸を回収する方法、前記回収方法に好適に用いられる核酸結合用担体および核酸回収キットの提供。 体積基準での粒度分布のD50が6~50μmであり、BJH法によって求めた細孔容積が0.3~5cm/gであり、BJH法によって求めた平均細孔径が60~500Åである多孔質シリカ粒子、または体積基準での粒度分布のD50が6~50μmであり、水銀ポロシメータにより求めた細孔容積が0.6~5cm/gであり、水銀ポロシメータにより求めた平均細孔径が200~5000Åである多孔質シリカ粒子、に核酸を結合させて複合体を形成し、前記複合体から前記核酸を溶出させ、回収する、核酸の回収方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)