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1. (WO2019033247) ESTABLISHMENT OF CELL LINE WITH STABLE AND HIGH EXPRESSION OF SISP1 GENE AND USE THEREOF
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/033247 International Application No.: PCT/CN2017/097430
Publication Date: 21.02.2019 International Filing Date: 14.08.2017
IPC:
C12N 15/79 (2006.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO.,LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室毛吉炎 MAO, Jiyan / Room 509, Building 1 Zhuguang Innovation Park, No.134 Zhuguang Road Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors:
毛吉炎 MAO, Jiyan; CN
Priority Data:
Title (EN) ESTABLISHMENT OF CELL LINE WITH STABLE AND HIGH EXPRESSION OF SISP1 GENE AND USE THEREOF
(FR) PRODUCTION D'UNE LIGNÉE CELLULAIRE À EXPRESSION STABLE ET ÉLEVÉE DU GÈNE SISP1 ET SON UTILISATION
(ZH) 一种 SISP1 基因稳定高表达细胞系的建立及其应用
Abstract:
(EN) Provided is a method for establishing a cell line with stable and high expression of the SISP1 gene, the method comprising: (1) inserting the coding sequence of the SISP1 gene into multiple cloning sites of an eukaryotic expression vector to obtain a recombinant expression vector; and (2) introducing the recombinant expression vector obtained in step 1 into a host cell, and screening the cells to obtain a cell line stably expressing the SISP1 gene. The cell line with stable and high expression of the SISP1 gene can be used for screening related drugs.
(FR) La présente invention concerne un procédé de production d'une lignée cellulaire à expression stable et élevée du gène SISP1, le procédé comprenant : (1) l'insertion de la séquence codante du gène SISP1 dans des sites de clonage multiples d'un vecteur d'expression eucaryote afin d'obtenir un vecteur d'expression recombinant; et (2) l'introduction du vecteur d'expression recombinant obtenu à l'étape (1) dans une cellule hôte, et le criblage des cellules afin d'obtenir une lignée cellulaire exprimant le gène SISP1 de manière stable. La lignée cellulaire à expression stable et élevée du gène SISP1 peut être utilisée pour cribler des médicaments associés.
(ZH) 提供了一种SISP1基因稳定高表达细胞系的建立方法,该方法包括:(1)将SISP1基因的编码序列插入真核表达载体的多克隆位点,得到重组表达载体;(2)将步骤1得到的重组表达载体导入宿主细胞,筛选得到稳定表达SISP1基因的细胞系。所述SISP1基因稳定高表达细胞系可用于相关药物的筛选。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)