Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2019033203) METHOD FOR DETERMINATION OF CELLULAR MRNA
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/033203 International Application No.: PCT/CA2018/050977
Publication Date: 21.02.2019 International Filing Date: 13.08.2018
IPC:
C12Q 1/6813 (2018.01) ,C12M 1/34 (2006.01) ,C12Q 1/6809 (2018.01) ,C12Q 1/6876 (2018.01) ,C12Q 1/6886 (2018.01) ,G06F 19/20 (2011.01)
[IPC code unknown for C12Q 1/6813]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
[IPC code unknown for C12Q 1/6809][IPC code unknown for C12Q 1/6876][IPC code unknown for C12Q 1/6886]
G PHYSICS
06
COMPUTING; CALCULATING; COUNTING
F
ELECTRIC DIGITAL DATA PROCESSING
19
Digital computing or data processing equipment or methods, specially adapted for specific applications
10
Bioinformatics, i.e. methods or systems for genetic or protein-related data processing in computational molecular biology
20
for hybridisation or gene expression, e.g. microarrays, sequencing by hybridisation, normalisation, profiling, noise correction models, expression ratio estimation, probe design or probe optimisation
Applicants:
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO [CA/CA]; 27 King's College Circle Toronto, Ontario M5S 1A1, CA
Inventors:
LABIB, Mahmoud; CA
SARGENT, Edward H.; CA
KELLEY, Shana O.; CA
Agent:
RIDOUT & MAYBEE LLP; 250 University Avenue, 5th Floor Toronto, Ontario M5H 3E5, CA
WONG, Christine; CA
Priority Data:
62/545,08114.08.2017US
Title (EN) METHOD FOR DETERMINATION OF CELLULAR MRNA
(FR) PROCÉDÉ DE DÉTERMINATION D’ARNM CELLULAIRE
Abstract:
(EN) Methods and systems for mRNA analysis and quantification of mRNA expression in cells are provided. An example method includes introducing a first capture probe and a second capture probe into the cells, the first capture probe and the second capture probe each configured to be complementary to a respective section of target mRNA within the cells, wherein binding of the first and second capture probes to the respective sections of the target mRNA results in tagging of the cells and causes the first and second capture probes to form clusters with each other. The first capture probe and the second capture probe are each bound to magnetic nanoparticles (MNPs) that, when trapped within the tagged cells, cause the tagged cells to be susceptible to magnetic forces. The method and system further include introducing the cells into a device configured to magnetically capture tagged cells.
(FR) La présente invention concerne des procédés et des systèmes d’analyse d’ARNm et de quantification de l’expression de l’ARNm dans des cellules. Un procédé donné à titre d'exemple comprend l’introduction d’une première sonde de capture et d’une seconde sonde de capture dans les cellules, la première sonde de capture et la seconde sonde de capture étant chacune configurées pour être complémentaires d’une section respective d’un ARNm cible dans les cellules, la liaison de la première et de la seconde sonde de capture aux sections respectives de l’ARNm cible entraînant le marquage des cellules et amenant la première et la seconde sonde de capture à former des amas l'une avec l'autre. La première sonde de capture et la seconde sonde de capture sont chacune liées à des nanoparticules magnétiques (MNP) qui, lorsqu’elles sont piégées à l’intérieur des cellules marquées, amènent les cellules marquées à être sensibles aux forces magnétiques. Le procédé et le système comprennent en outre l’introduction des cellules dans un dispositif configuré pour capturer magnétiquement les cellules marquées.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)