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1. (WO2019032762) METHODS TO IMPROVE THE SEQUENCING OF POLYNUCLEOTIDES WITH BARCODES USING CIRCULARISATION AND TRUNCATION OF TEMPLATE
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/032762 International Application No.: PCT/US2018/045893
Publication Date: 14.02.2019 International Filing Date: 09.08.2018
IPC:
C12Q 1/6869 (2018.01) ,C12Q 1/6806 (2018.01) ,C12Q 1/6804 (2018.01) ,C12N 15/10 (2006.01)
[IPC code unknown for C12Q 1/6869][IPC code unknown for C12Q 1/6806][IPC code unknown for C12Q 1/6804]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
ROOTPATH GENOMICS, INC. [US/US]; 43 Charles Street, Suite 2 Boston, Massachusetts 02114, US
Inventors:
PORTER, Ely; US
CHEN, Xi; US
Agent:
SCHOENHARD, Amy E.L.; US
MCNEILL, Rebecca M.; US
BAUR, Amelia F.; US
SCARR, Rebecca B.; US
HENNINGER, Mary R.; US
DOHERTY, Elizabeth A.; US
BREIER, Adam M.; US
HERZFELD, Deborah M.; US
PRYBYLOWSKI, Kate; US
LEE, Victoria; US
RACHINSKY, Tara; US
CHO, Hojung; US
Priority Data:
62/543,61210.08.2017US
Title (EN) METHODS TO IMPROVE THE SEQUENCING OF POLYNUCLEOTIDES WITH BARCODES USING CIRCULARISATION AND TRUNCATION OF TEMPLATE
(FR) PROCÉDÉS POUR AMÉLIORER LE SÉQUENÇAGE DE POLYNUCLÉOTIDES À L'AIDE DE CODES-BARRES EN UTILISANT UNE CIRCULARISATION ET UNE TRONCATURE DE MATRICE
Abstract:
(EN) The application provides methods of generating truncated and barcoded nucleic acid molecules from at least two target polynucleotide sequences, each from distinct biological particles, whereby the barcoded nucleic acids are circularised and subsequently partial linear fragments for amplification and seqeuncing are generated.
(FR) L'invention concerne des procédés de génération de molécules d'acide nucléique tronquées et à code-barres à partir d'au moins deux séquences polynucléotidiques cibles, chacune provenant de particules biologiques distinctes, par lesquels les acides nucléiques à code-barres sont circularisés, cela étant suivi de la génération de fragments linéaires partiels pour l'amplification et le séquençage.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)