Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2019032345) USE OF MAGNETIC CELLS TO MANIPULATE NON-MAGNETIC CELLS
Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

CLAIMS:

1) A method of isolating target cells from a mixture of cells, comprising:

a) combining a mixture of cells comprising target cells and non-target cells with magnetized display cells that display one or more ligands on their surfaces, said ligands specific for a binding partner on a surface of said target cells;

b) allowing said ligand to bind to said binding partner to produce magnetized display cells bound to said target cells;

c) applying a magnetic field to collect said magnetized display cells bound to said target cells; and

d) washing away non-target cells, thereby isolating said target cells.

2) The method of claim 1, wherein said ligands comprising antigen binding sites and said binding partner comprises an antigen that is recognized by said antigen binding sites.

3) The method of claim 1-2, wherein said magnetized display cells are fibroblasts.

4) The method of claim 1-2, wherein said magnetized display cells are from a fibroblast derived cell line.

5) The method of claim 1-4, wherein said target cells and said magnetized display cells are human.

6) The method of claims 1-5, comprising the further step of 3D culturing said magnetized display cells bound to said target cells.

7) The method of claims 1-5, comprising the further step of 3D culturing said

magnetized display cells bound to said target cells in a magnetic field such that said magnetized display cells bound to said target cells are levitated.

8) The method of claims 1-7, wherein said magnetized display cells are provided by treating cells displaying one or more ligands with magnetic nanoparticles.

9) The method of claim 8, wherein said magnetic nanoparticles are introduced into said cells displaying one or more ligands by blasting, injection, electroporation, magnetic pressure, hydrogels, or cationic liposomes.

10) The method of claim 8, wherein said magnetic nanoparticles are introduced into said cells displaying one or more ligands with a composition comprising:

a) a negatively charged nanoparticle;

b) a positively charged nanoparticle; and

c) a support molecule,

d) wherein one of said negatively charged nanoparticle or positively charged

nanoparticle is a magnetically responsive element or compound, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure.

11) A method of isolating target cells from a mixture of cells, comprising:

a) combining cells displaying one or more antigen binding site(s) on their surfaces with a composition to form magnetized display cells, said composition comprising:

i) a negatively charged nanoparticle;

ii) a positively charged nanoparticle; and

iii) a support molecule;

iv) wherein one of said negatively charged nanoparticle or positively charged

nanoparticle is a magnetically responsive element or compound, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure;

b) combining a mixture of cells comprising target cells and non-target cells with said magnetized display cells;

c) allowing said antigen binding site(s) to bind to one or more target cell(s) displaying an antigen that is recognized by said antigen binding site(s);

d) applying a magnetic field to collect magnetized display cells bound to said target cells; and

e) washing away non-target cells, thereby isolating magnetized display cells bound to said target cells.

12) The method of claim 11, comprising the further step of separating the magnetized display cells from the target cells.

13) The method of claim 11, comprising the further step of 3D culturing said isolating magnetized display cells bound to said target cells in a magnetic field sufficient to levitate said isolating magnetized cells bound to said target cells.

14) The method of claims 11-13, a) wherein the support molecule comprises peptides, polysaccharides, nucleic acids, polymers, poly-lysine, fibronectin, collagen, laminin, BSA, hyaluronan, glycosaminoglycan, anionic, non-sulfated glycosaminoglycan, gelatin, nucleic acid, extracellular matrix protein mixtures, antibody, or mixtures or derivatives thereof, b) wherein said negatively charged nanoparticle is a gold nanoparticle, and c) wherein said positively charged nanoparticle is an iron oxide nanoparticle.

15) A method of manipulating target cells, comprising:

a) combining target cells with magnetized display cells that display one or more ligands on their surfaces, said ligands for specifically binding a target molecule on a surface of said target cells;

b) allowing said ligands to bind to said target molecule to produce magnetized display cells bound to said target cells;

c) applying a magnetic field to collect said magnetized display cells bound to said target cells, thereby allowing manipulation of said target cells.

16) A magnetic cell complex comprising:

a) at least one magnetized display cell displaying at least one ligand on a surface of the at least one magnetized display cell; and

b) at least one target cell having at least one binding partner on a surface of the at least one target cell,

wherein said at least one ligand on the surfaces of the at least one magnetized display cell binds said binding partner on the surface of the at least one target cell so as to form the magnetic cell complex.

17) The magnetic cell complex of claim 16, wherein said at least one ligand comprises antigen binding site(s) and said at least one binding partner comprises an antigen that is recognized by said antigen binding site(s).

18) The magnetic cell complex of claims 16-17, wherein the at least one magnetized display cell comprises magnetic nanoparticles.