Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2019032054) METHOD FOR SCREENING SPLICING VARIANTS OR EVENTS
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/032054 International Application No.: PCT/SG2018/050408
Publication Date: 14.02.2019 International Filing Date: 13.08.2018
IPC:
C12Q 1/6813 (2018.01) ,C12N 15/113 (2010.01)
[IPC code unknown for C12Q 1/6813]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
Applicants:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower Singapore 138632, SG
Inventors:
WEE, Keng Boon; SG
Agent:
AMICA LAW LLC; 77 Robinson Road #22-01 Robinson 77 Singapore 068896, SG
Priority Data:
10201706585X11.08.2017SG
Title (EN) METHOD FOR SCREENING SPLICING VARIANTS OR EVENTS
(FR) PROCÉDÉ DE CRIBLAGE DE VARIANTS OU D'ÉVÉNEMENTS D'ÉPISSAGE
Abstract:
(EN) The present invention relates to a high-throughput method of screening splicing variants of target genes as drug targets or for characterisation of their biological functions. The disclosure provides a method for the screening of splicing variants, comprising: (a) providing a first antisense oligonucleotide capable of inducing a first splice event on the target gene to express a first splicing variant, and a second antisense oligonucleotide capable of inducing a second splice event on the target gene to express a second splicing variant; (b) hybridising the first and second antisense oligonucleotides to a pre-mRNA of the target gene; and (c) characterising the effect of the splice event. In one embodiment, the first antisense oligonucleotide switches the splice event that expresses the second splicing variant towards one that expresses the first splicing variant, while the second antisense oligonucleotide switches the splice event that expresses the first splicing variant towards one that expresses the second splicing variant.
(FR) La présente invention concerne un procédé à haut rendement de criblage de variants d'épissage de gènes cibles en tant que cibles de médicament ou pour caractériser leurs fonctions biologiques. L'invention concerne un procédé de criblage de variants d'épissage consistant (a) à fournir un premier oligonucléotide antisens capable d'induire un premier événement d'épissage sur le gène cible pour exprimer un premier variant d'épissage, et un second oligonucléotide antisens capable d'induire un second événement d'épissage sur le gène cible pour exprimer un second variant d'épissage; (b) à hybrider les premier et second oligonucléotides antisens à un pré-ARNm du gène cible; et (c) à caractériser l'effet de l'événement d'épissage. Dans un mode de réalisation, le premier oligonucléotide antisens fait passer l'événement d'épissage qui exprime le second variant d'épissage vers un qui exprime le premier variant d'épissage, tandis que le second oligonucléotide antisens fait passer l'événement d'épissage qui exprime le premier variant d'épissage vers un qui exprime le second variant d'épissage.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)