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1. (WO2019031500) IMMORTALIZED SWEAT GLAND MYOEPITHELIAL CELL
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/031500 International Application No.: PCT/JP2018/029594
Publication Date: 14.02.2019 International Filing Date: 07.08.2018
IPC:
C12N 5/10 (2006.01) ,C12N 5/074 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
074
Adult stem cells
Applicants:
株式会社マンダム MANDOM CORPORATION [JP/JP]; 大阪府大阪市中央区十二軒町5番12号 5-12, Juniken-cho, Chuo-ku, Osaka-shi, Osaka 5408530, JP
国立大学法人大阪大学 OSAKA UNIVERSITY [JP/JP]; 大阪府大阪府吹田市山田丘1番1号 1-1, Yamadaoka, Suita-shi, Osaka 5650871, JP
Inventors:
早川 智久 HAYAKAWA Tomohisa; JP
倉田 隆一郎 KURATA Ryuichiro; JP
藤田 郁尚 FUJITA Fumitaka; JP
岡田 文裕 OKADA Fumihiro; JP
関口 清俊 SEKIGUCHI Kiyotoshi; JP
Agent:
赤松 善弘 AKAMATSU Yoshihiro; JP
Priority Data:
2017-15450309.08.2017JP
Title (EN) IMMORTALIZED SWEAT GLAND MYOEPITHELIAL CELL
(FR) CELLULE MYOÉPITHÉLIALE IMMORTALISÉE DE GLANDES SUDORIPARES
(JA) 不死化汗腺筋上皮細胞
Abstract:
(EN) An immortalized sweat gland myoepithelial cell which expresses α-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial cells, said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on the surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.
(FR) La présente invention concerne une cellule myoépithéliale immortalisée de glandes sudoripares qui exprime l'α-SMA et la pan-cytokératine et a une capacité de formation de sphères après une sous-culture au moins 5 fois. L'invention concerne un procédé de production de cellules myoépithéliales immortalisées de glandes sudoripares, ledit procédé comprenant : le transfert d'un gène immortalisant dans les cellules, tout en cultivant une structure cellulaire, les cellules myoépithéliales de glandes sudoripares étant exposées sur la surface, dans un état de suspension dans un milieu ; puis la culture de la structure transgénique ainsi obtenue dans un état de suspension dans le milieu pour obtenir ainsi des cellules myoépithéliales immortalisées de glandes sudoripares.
(JA) α-SMAおよびパンサイトケラチンを発現し、少なくとも5回の継代後にスフィア形成能を有する不死化汗腺筋上皮細胞、汗腺筋上皮細胞が表面に露出している細胞構造体を培地中に浮遊させた状態で培養しながら、不死化遺伝子を該細胞に導入し、得られた遺伝子導入体を培地中に浮遊させた状態で培養して不死化汗腺筋上皮細胞を得る不死化汗腺筋上皮細胞の製法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)