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1. (WO2019030755) IMPROVED ENDOINULINASES
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/030755 International Application No.: PCT/IL2018/050872
Publication Date: 14.02.2019 International Filing Date: 07.08.2018
IPC:
C12N 9/24 (2006.01) ,C12N 15/01 (2006.01) ,C07H 3/06 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01
Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3
Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06
Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
Applicants:
MIGAL APPLIED RESEARCH, LTD. [IL/IL]; POB 831 1101602 Kiryat Shmona, IL
Inventors:
YADID, Itamar; IL
COHEN, Rami; IL
AFRIAT-JURNOU, Livnat; IL
Agent:
SCHWARZ, Mikael; IL
SCHWARZ, Mikael; IL
Priority Data:
62/541,82007.08.2017US
Title (EN) IMPROVED ENDOINULINASES
(FR) ENDO-INULINASES AMÉLIORÉES
Abstract:
(EN) A modified endoinulinase is provided, comprising modified wild-type T. purpuregenus endoinulinase, or a functional fragment thereof, in which an amino acid residue at each one of one or more positions corresponding to 128, 316, 344, 350 or 504 of wild-type T. purpuregenus endoinulinase is substituted, wherein: (i) a tyrosine residue corresponding to Y128 is substituted with H, K or R; a glutamate residue corresponding to E344 is substituted with K, H or R; and a threonine residue corresponding to T504 is substituted with M, S or Y; and optionally an alanine residue corresponding to A316 is substituted with T, S, C or M; (ii) a tyrosine residue corresponding to Y128 is substituted with H, K or R; a glutamate residue corresponding to E344 is substituted with K, H or R; a threonine residue corresponding to T504 is substituted with M, S or Y; and a glutamine residue corresponding to Q350 is substituted with L, G, A, V or I; or (iii) a tyrosine residue corresponding to Y128 is substituted with H, K or R.
(FR) L'invention concerne une endo-inulinase modifiée, comprenant une endoinulinase T. purpuregenus de type sauvage modifiée, ou un fragment fonctionnel de celle-ci, dans laquelle un résidu d'acide aminé à chaque position d'une ou de plusieurs positions correspondant à 128, 316, 344, 350 ou 504 de endoinulinase T. purpuregenus. de type sauvage est substitué : (i) un résidu tyrosine correspondant à Y128 est substitué par H, K ou R; un résidu glutamate correspondant à E344 est substitué par K, H ou R; et un résidu thréonine correspondant à T504 est substitué par M, S ou Y; et éventuellement un résidu alanine correspondant à A316 est substitué par T, S, C ou M; (ii) un résidu tyrosine correspondant à Y128 est substitué par H, K ou R; un résidu glutamate correspondant à E344 est substitué par K, H ou R; un résidu thréonine correspondant à T504 est substitué par M, S ou Y; et un résidu glutamine correspondant à Q350 est substitué par L, G, A, V ou I; ou (iii) un résidu tyrosine correspondant à Y128 est substitué par H, K ou R.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)