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1. (WO2019030519) DETECTION OF CARBAPENEMASE-PRODUCING ENTEROBACTERIALES, PSEUDOMONAS AND ACINETOBACTER SPECIES USING A CHROMOGEN
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Pub. No.: WO/2019/030519 International Application No.: PCT/GB2018/052255
Publication Date: 14.02.2019 International Filing Date: 07.08.2018
IPC:
C12Q 1/04 (2006.01) ,C12Q 1/10 (2006.01) ,C12Q 1/25 (2006.01) ,C12Q 1/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
10
Enterobacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
25
involving enzymes not classifiable in groups C12Q1/26-C12Q1/7097
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
Applicants:
MAST GROUP LIMITED [GB/GB]; Mast House Derby Road Liverpool Merseyside L20 1EA, GB
Inventors:
HOBSON, Jonathan Anthony; GB
COLEBORN, Matilda May; GB
DAVIES, Mya; GB
ANYAKWO, Andrew Chuka; GB
Agent:
REES, Kerry; GB
Priority Data:
1712671.507.08.2017GB
Title (EN) DETECTION OF CARBAPENEMASE-PRODUCING ENTEROBACTERIALES, PSEUDOMONAS AND ACINETOBACTER SPECIES USING A CHROMOGEN
(FR) DÉTECTION D’ESPÈCES PSEUDOMONAS ET ACINETOBACTER ENTÉROBACTÉRIALES PRODUISANT DES CARBAPÉNÉMASES À L'AIDE D'UN CHROMOGÈNE
Abstract:
(EN) The present application relates to a method for detecting the presence of carbapenemase- producing Enterobacteriales, Pseudomonas and Ancinetobacter in a sample, comprising; providing a sample suspected of having carbapenemase-producing Enterobacteriales, Pseudomonas or Acinetobacter; reacting the sample with a solution of (7R)-7-[(z)-2-(2- aminothiazol-4-yl)-(Z)-2(methoxyimino)acetamide]-3-(2,4-dinitrostyryl)-3-cephem-4 carboxylic acid) or a salt thereof, and detecting a color change in the reaction medium when carbapenemase-producing Enterobacteriales, Pseudomonas or Acinetobacter are present in the test sample.
(FR) La présente invention concerne un procédé de détection de la présence d’entérobactériales produisant des carbapénémases, Pseudomonas et Acinetobacter dans un échantillon, comprenant : la préparation d’un échantillon suspecté de présenter des entérobactériales produisant des carbapénémases, Pseudomonas ou Acinetobacter ; la réaction de l’échantillon avec une solution de (7R)-7-[(z)-2-(2-aminothiazol-4-yl)-(Z)-2(méthoxyimino)acétamide]-3-(2,4-dinitrostyryl)-3-cephem-acide 4-carboxylique) ou un sel de ce dernier, et la détection d’un changement de couleur dans le milieu réactionnel lorsque des entérobactériales produisant des carbapénémases, Pseudomonas ou Acinetobacter sont présentes dans l’échantillon de test.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)