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1. (WO2019030373) Integration sites in CHO cells
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/030373 International Application No.: PCT/EP2018/071733
Publication Date: 14.02.2019 International Filing Date: 10.08.2018
IPC:
C12N 15/90 (2006.01) ,C07K 16/00 (2006.01) ,C12N 9/22 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
Applicants:
BOEHRINGER INGELHEIM INTERNATIONAL GMBH [DE/DE]; Binger Str. 173 55216 Ingelheim am Rhein, DE
Inventors:
MUELLER, Markus; DE
SCHAUB, Jochen; DE
BERNLOEHR, Christian; DE
KOENITZER, Jennifer; DE
Agent:
WALLINGER RICKER SCHLOTTER TOSTMANN; Patent- und Rechtsanwälte Partnerschaft mbB Zweibrückenstraße 5-7 80331 München, DE
Priority Data:
17185988.711.08.2017EP
Title (EN) Integration sites in CHO cells
(FR) SITES D’INTÉGRATION DANS DES CELLULES CHO
Abstract:
(EN) The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.
(FR) La présente invention concerne l'identification d'un site d'intégration génomique pour des polynucléotides hétérologues dans des cellules Ovariennes de Hamster Chinois (CHO) conduisant à une production d'ARN et/ou de protéines élevée. Plus spécifiquement, l'invention concerne des cellules CHO comprenant au moins un polynucléotide hétérologue intégré de manière stable dans le groupe de gènes S100A du génome CHO et des méthodes pour la production desdites cellules CHO. En outre, l'invention concerne une méthode de production d'une protéine d'intérêt à l'aide de ladite cellule CHO et l'utilisation de ladite cellule CHO pour produire une protéine d'intérêt à un rendement élevé. L'intégration à l'intérieur de ces régions cibles spécifiques conduit à la production fiable, stable et à haut rendement d'un ARN et/ou d'une protéine d'intérêt, codé par le polynucléotide hétérologue.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)