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1. (WO2019030306) IN VITRO ISOLATION AND ENRICHMENT OF NUCLEIC ACIDS USING SITE-SPECIFIC NUCLEASES
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/030306 International Application No.: PCT/EP2018/071557
Publication Date: 14.02.2019 International Filing Date: 08.08.2018
IPC:
C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
DEPIXUS [FR/FR]; 3-5 IMPASSE REILLE 75014 PARIS, FR
Inventors:
OUELLET, Jimmy; FR
MALUENDA, Jerôme; FR
Agent:
REGIMBEAU; 20, rue de Chazelles 75847 PARIS CEDEX 17, FR
Priority Data:
17306055.908.08.2017EP
Title (EN) IN VITRO ISOLATION AND ENRICHMENT OF NUCLEIC ACIDS USING SITE-SPECIFIC NUCLEASES
(FR) ISOLEMENT ET ENRICHISSEMENT IN VITRO D'ACIDES NUCLÉIQUES À L'AIDE DE NUCLÉASES SPÉCIFIQUES À UN SITE
Abstract:
(EN) The present invention relates to a method for the isolation of a target nucleic acid region. In particular, said method comprises the steps of contacting a population of nucleic acid molecules with at least one Type II Cas protein -gRNA complex, wherein said gRNA comprises a guide segment that is complementary to the sequence comprised in the target region of at least one nucleic acid molecule, thereby forming a Type II Cas protein-gRNA-nucleic acid complex, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, and isolating the target nucleic acid region from the Type II Cas protein-gRNA-nucleic acid complex.
(FR) La présente invention concerne une méthode d'isolement d'une région d'acide nucléique cible. En particulier, ladite méthode comprend les étapes consistant à mettre en contact une population de molécules d'acide nucléique avec au moins un complexe protéine Cas de type II-ARNg, ledit ARNg comprenant un segment de guidage qui est complémentaire de la séquence comprise dans la région cible d'au moins une molécule d'acide nucléique, ce qui permet de former un complexe protéine Cas de type II-ARNg-acide nucléique, mettre en contact la population de molécules d'acide nucléique avec au moins une enzyme ayant une activité exonucléase, et isoler la région d'acide nucléique cible à partir du complexe protéine Cas de type II-ARNg-acide nucléique.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)