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1. (WO2019030069) USE OF CONSTITUTIVELY ACTIVE VARIANTS OF GROWTH FACTOR RECEPTORS AS SELECTION MAKERS FOR THE GENERATION OF STABLE PRODUCER CELL LINES
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2019/030069 International Application No.: PCT/EP2018/070829
Publication Date: 14.02.2019 International Filing Date: 01.08.2018
IPC:
C12N 15/85 (2006.01) ,C12N 15/113 (2010.01) ,C07K 14/71 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
71
for growth factors; for growth regulators
Applicants:
CEVEC PHARMACEUTICALS GMBH [DE/DE]; Gottfried-Hagen-Str. 62 51105 Köln, DE
Inventors:
FAUST, Nicole; DE
WISSING, Silke; DE
STREMPEL, Nikola; DE
Agent:
MÜLLER-BORÉ & PARTNER PATENTANWÄLTE PARTG MBB; Friedenheimer Brücke 21 80639 München, DE
Priority Data:
17001356.908.08.2017EP
Title (EN) USE OF CONSTITUTIVELY ACTIVE VARIANTS OF GROWTH FACTOR RECEPTORS AS SELECTION MAKERS FOR THE GENERATION OF STABLE PRODUCER CELL LINES
(FR) UTILISATION DE VARIANTS CONSTITUTIVEMENT ACTIFS DE RÉCEPTEURS DU FACTEUR DE CROISSANCE EN TANT QUE MARQUEURS DE SÉLECTION POUR LA GÉNÉRATION DE LIGNÉES CELLULAIRES PRODUCTRICES STABLES
Abstract:
(EN) The present invention relates to methods for the selection of a stable producer cell line expressing one or more protein(s) of interest (POIs), comprising the stable transfection of cells with a gene encoding a constitutively active variant of a growth factor receptor, respective methods for the expression of one or more POIs in such stable producer cell lines, and uses of a constitutively active variant of a growth factor receptor as selection marker in the cultivation of cells.
(FR) La présente invention concerne des procédés de sélection d'une lignée cellulaire productrice stable exprimant une ou plusieurs protéines(s) d'intérêt (POI), comprenant la transfection stable de cellules avec un gène codant pour une variante constitutivement active d'un récepteur de facteur de croissance, des procédés respectifs pour l'expression d'un ou de plusieurs POI dans de telles lignées cellulaires productrices stables, et les utilisations d'un variant constitutivement actif d'un récepteur de facteur de croissance comme marqueur de sélection dans la culture de cellules.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)