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1. (WO2019028450) METHOD FOR PRODUCING THERAPEUTIC EXOSOMES FROM NANOELECTROPORATION AND OTHER NON-ENDOCYTIC CELL TRANSFECTION
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Pub. No.: WO/2019/028450 International Application No.: PCT/US2018/045333
Publication Date: 07.02.2019 International Filing Date: 06.08.2018
IPC:
A61K 9/00 (2006.01) ,A61M 37/00 (2006.01) ,A61M 5/158 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9
Medicinal preparations characterised by special physical form
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
M
DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
37
Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
M
DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
5
Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm rests
14
Infusion devices, e.g. infusing by gravity; Blood infusion; Accessories therefor
158
Needles
Applicants:
OHIO STATE INNOVATION FOUNDATION [US/US]; 1524 North Hight Street Columbus, OH 43201, US
Inventors:
LEE, Ly, James; US
SHI, Junfeng; US
YANG, Zhaogang; US
Agent:
STANDLEY, Jeffrey, S.; US
GRANT, Stephen, L.; US
Priority Data:
62/541,15704.08.2017US
Title (EN) METHOD FOR PRODUCING THERAPEUTIC EXOSOMES FROM NANOELECTROPORATION AND OTHER NON-ENDOCYTIC CELL TRANSFECTION
(FR) PROCÉDÉ DE PRODUCTION D'EXOSOMES THÉRAPEUTIQUES À PARTIR DE NANOÉLECTROPORATION ET AUTRE TRANSFECTION DE CELLULE NON ENDOCYTIQUE
Abstract:
(EN) Therapeutic extracellular vesicles (EVs) containg high copies of functional nucleic acids and other biomolecules are produced in large quantities by laying donor cells on a surface of a chip, adding various plasmids, other transfection vectors and their combinations to a buffer on the chip, applying a pulsulatic electric field across the cells laid on top of the chip surface and plasmids/vectors buffer solution below the chip surface, and collecting the EVs secreted by the transfected cells. The chip surface has a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed on it, capable of handling large quantities of the donor cells. The buffer is adapted for receiving plasmids and other transfection vectors.
(FR) Selon l'invention, des vésicules extracellulaires (EV) thérapeutiques contenant des copies élevées d'acides nucléiques fonctionnels et d'autres biomolécules sont produites en grandes quantités par la disposition de cellules donneuses sur la surface d'une puce, l'ajout de divers plasmides, d'autres vecteurs de transfection et de leurs combinaisons à un tampon sur la puce, l'application d'un champ électrique pulsé à travers les cellules disposées sur de la surface de la puce et les plasmides/la solution tampon de vecteurs sous la surface de la puce, et la collecte des EV secrétées par les cellules transfectées. La surface de la puce inclut une biopuce à électroporation (NEP) tridimensionnelle (3D) formée sur celle-ci, qui est capable de gérer de grandes quantités des cellules donneuses. Le tampon est conçu pour recevoir des plasmides et d'autres vecteurs de transfection.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)